Gametocytes from K13 Propeller Mutant Plasmodium falciparum Clinical Isolates Demonstrate Reduced Susceptibility to Dihydroartemisinin in the Male Gamete Exflagellation Inhibition Assay.

Antimicrobial Agents and Chemotherapy
Sonia LozanoJanneth Rodrigues

Abstract

Mutations in the kelch propeller domain (K13 propeller) of Plasmodium falciparum parasites from Southeast Asia are associated with reduced susceptibility to artemisinin. We exposed in vitro-cultured stage V gametocytes from Cambodian K13 propeller mutant parasites to dihydroartemisinin and evaluated the inhibition of male gamete formation in an in vitro exflagellation inhibition assay (EIA). Gametocytes with the R539T and C580Y K13 propeller alleles were less susceptible to dihydroartemisinin and had significantly higher 50% inhibitory concentrations (IC50s) than did gametocytes with wild-type alleles.

References

Dec 10, 2008·The New England Journal of Medicine·Harald NoedlUNKNOWN Artemisinin Resistance in Cambodia 1 (ARC1) Study Consortium
Jul 31, 2009·The New England Journal of Medicine·Arjen M DondorpNicholas J White
Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sophie H AdjalleyDavid A Fidock
Sep 4, 2012·The Lancet Infectious Diseases·Chanaki AmaratungaRick M Fairhurst
Dec 20, 2013·Nature·Frédéric ArieyDidier Ménard
May 29, 2014·Antimicrobial Agents and Chemotherapy·Chanaki AmaratungaRick M Fairhurst
Jul 31, 2014·The New England Journal of Medicine·Elizabeth A AshleyUNKNOWN Tracking Resistance to Artemisinin Collaboration (TRAC)

❮ Previous
Next ❯

Citations

Jun 22, 2019·Biology Open·Takeshi Q TanakaFuyuki Tokumasu
Nov 4, 2020·Antimicrobial Agents and Chemotherapy·Kathrin WitmerJake Baum
Nov 9, 2019·Trends in Parasitology·Arthur M TalmanFrédéric Ariey
Jul 17, 2021·Current Opinion in Infectious Diseases·Mehul DhordaArjen M Dondorp

❮ Previous
Next ❯

Methods Mentioned

BETA
RSA

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.