PMID: 6112752Mar 1, 1981Paper

gamma-Aminobutyric acid and benzodiazepine receptors: copurification and characterization

Proceedings of the National Academy of Sciences of the United States of America
M Gavish, S H Snyder

Abstract

gamma-Aminobutyric acid (GABA) and benzodiazepine receptors have been solubilized and purified by procedures such as gel filtration, ion-exchange, lectin, and affinity chromatographies. All of these procedures enhance the specific activity of each receptor to a similar extent. The drug specificities of [3H]muscimol and [3H]flunitrazepam binding sites are the same after extensive purification by affinity chromatography compared to the membrane bound and initially solubilized receptors. GABA and chloride stimulation of benzodiazepine binding is retained in pure receptors. Two bands are covalently labeled with [3H]flunitrazepam after ultraviolet irradiation of the purified receptor. The persistent association of GABA, benzodiazepine, and chloride recognition sites after extensive purification suggests that they may be part of a single macromolecular complex.

References

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Citations

Dec 17, 1981·European Journal of Pharmacology·S M PaulP Skolnick
Jun 3, 1983·European Journal of Pharmacology· Korneyev AYa
Nov 25, 1983·European Journal of Pharmacology·P Schoch, H Möhler
Jul 13, 1984·European Journal of Pharmacology·E H WongR W Olsen
Dec 1, 1983·Neuropharmacology·R F Squires
Dec 1, 1983·Neuropharmacology·D W Gallager, J F Tallman
Feb 1, 1986·Brain Research·J L MaderdrutR W Oppenheim
Jul 1, 1986·Neuroscience Research·Y Yoneda, K Ogita
Aug 1, 1991·Brain Research. Molecular Brain Research·V M Gehle, K Sumikawa
Jan 1, 1983·Neurochemistry International·R Sherman-Gold
Jan 1, 1987·Neurochemistry International·I YamadaH Yoshida
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M M LoS H Snyder
Sep 6, 2001·Hormones and Behavior·A G Mensah-NyaganH Vaudry
Sep 1, 1985·Neurochemical Research·J P RayA L de Blas
May 1, 1985·Journal of Neurochemistry·E I TietzH C Rosenberg

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