Gamma-Delta T Lymphocytes in the Diagnostic Approach of Coeliac Disease

Journal of Clinical Gastroenterology
Petula NijeboerGerd Bouma

Abstract

To validate cut-off values of CD3 T-cell receptor gamma-delta chain (TCRγδ) intraepithelial lymphocyte (IEL) in the (differential) diagnosis of celiac disease (CD). CD is characterized by an increase in gamma-delta IEL (CD3TCRγδ IEL). Percentages were determined by flow cytometric analysis of IELs from small bowel biopsies in 213 CD and 13 potential CD (PCD) patients and in total 112 controls. A cut-off value for percentages of CD3TCRγδ IEL to differentiate active CD and controls was obtained from a receiver operating characteristic curve and implemented in controls and PCD patients. Percentage of CD3TCRγδ IEL was significantly increased in the majority of CD patients, irrespective of the presence of villous atrophy. A cut-off value of 14% for CD3TCRγδ IEL resulted in 66.3% sensitivity and 96.6% specificity for CD diagnosis (area under the curve, 88.6%). A percentage of ≥14% CD3TCRγδ IEL has a high specificity for CD diagnosis and can be of diagnostic help in cases where diagnosis is not straightforward.

References

Jul 1, 1991·Clinical and Experimental Immunology·J SpencerJ A Walker-Smith
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaping ChenRichard Boismenu
Jun 24, 2003·The American Journal of Gastroenterology·Teea T JärvinenPekka Collin
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyoko Inagaki-OharaAkihiko Yoshimura
Oct 26, 2007·The New England Journal of Medicine·Peter H R Green, Christophe Cellier
Nov 21, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Wieke H M VerbeekChris J J Mulder
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anisa S IsmailLora V Hooper
Mar 10, 2010·Nature Reviews. Gastroenterology & Hepatology·Greetje J TackChris J J Mulder
Sep 14, 2010·Journal of Immunological Methods·Francisco Leon
Jan 29, 2011·World Journal of Gastroenterology : WJG·Greetje J TackChris J J Mulder
Jul 13, 2012·Mucosal Immunology·M SarraG Monteleone
Apr 9, 2016·Anatomical Science International·Masaki Ogata, Tsunetoshi Itoh
Sep 25, 2016·Auto- Immunity Highlights·M Sánchez-CastañonMarcos López-Hoyos

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Citations

Jun 26, 2020·Journal for Immunotherapy of Cancer·Yousef R BadranMichael Dougan
Aug 1, 2020·Frontiers in Immunology·Margaret R DunneConleth Feighery
Mar 18, 2020·Allergy·Marcela de Lima MoreiraSidonia B G Eckle
Apr 4, 2021·International Journal of Molecular Sciences·Johannes WolfHans Binder
Aug 2, 2021·Clinical and Translational Gastroenterology·Roy L J van WanrooijGerd Bouma

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Methods Mentioned

BETA
flow cytometry
biopsy
biopsies

Software Mentioned

SPSS
Cell quest

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