PMID: 3997870Jun 25, 1985

gamma-Glutamyl amino acids. Transport and conversion to 5-oxoproline in the kidney

The Journal of Biological Chemistry
R J Bridges, A Meister

Abstract

Transport of gamma-glutamyl amino acids, a step in the proposed glutathione-gamma-glutamyl transpeptidase-mediated amino acid transport pathway, was examined in mouse kidney. The transport of gamma-glutamyl amino acids was demonstrated in vitro in studies on kidney slices. Transport was followed by measuring uptake of 35S after incubation of the slices in media containing gamma-glutamyl methionine [35S]sulfone. The experimental complication associated with extracellular conversion of the gamma-glutamyl amino acid to amino acid and uptake of the latter by slices was overcome by using 5-oxoproline formation (catalyzed by intracellular gamma-glutamyl-cyclotransferase) as an indicator of gamma-glutamyl amino acid transport. This method was also successfully applied to studies on transport of gamma-glutamyl amino acids in vivo. Transport of gamma-glutamyl amino acids in vitro and in vivo is inhibited by several inhibitors of gamma-glutamyl transpeptidase and also by high extracellular levels of glutathione. This seems to explain urinary excretion of gamma-glutamylcystine by humans with gamma-glutamyl transpeptidase deficiency and by mice treated with inhibitors of this enzyme. Mice depleted of glutathione by treatment with buthionin...Continue Reading

Related Concepts

Extracellular
Structure-Activity Relationship
Amino Acids, I.V. solution additive
Gamma-glutamyl transferase
Protoplasm
Pyroglutamate
Uptake
Buthionine Sulfoximine
Kidney
Glutathione Measurement

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