gamma-Glutamyltransferase from human hepatoma tissue in comparison with normal liver enzyme

Clinica Chimica Acta; International Journal of Clinical Chemistry
H YamamotoY Yamamura

Abstract

gamma-Glutamyltransferase was solubilized from human hepatoma tissues by bromelain treatment, and some of its properties were compared with those of the normal adult liver enzyme. An electrophoretic study showed a slightly different mobility between the two enzymes before and after neuraminidase treatment. The hepatoma tissue enzyme was distinguished from the normal liver enzyme by decreased affinity to Con A. However, the enzymes from the two sources were found to be very similar or identical with respect to molecular weight, Michaelis constant, pH optimum, thermostability, effect of various L-amino acids as acceptors, behavior to divalent cations or ethylenediaminetetraacetate, inhibition by urea or sodium dodecyl sulfate, and immunological properties. These results suggest that the hepatoma tissue gamma-glutamyltransferase is largely due to altered glycosylation of this glycoprotein in hepatoma cells.

References

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Citations

Dec 30, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·N E Huseby, T J Eide
Mar 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·F KanemitsuJ Mizushima
May 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Wellman-BednawskaG Siest
Feb 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·J R DelangheR J Wieme
Dec 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·C YoshikawaJ Ohkawa
Oct 31, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·C MassoubreJ Harb
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R E Smith
Jan 1, 1993·The Journal of Dermatology·Y TakamiM Takahashi
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Robert E Smith
Jul 1, 1985·Annals of Clinical Biochemistry·K D HammondD Piesas

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