gamma-Hydroxybutyrate binds to the synaptic site recognizing succinate monocarboxylate: a new hypothesis on astrocyte-neuron interaction via the protonation of succinate

Journal of Neuroscience Research
T MolnárJulianna Kardos

Abstract

Succinate (SUC), a citrate (CIT) cycle intermediate, and carbenoxolone (CBX), a gap junction inhibitor, were shown to displace [3H]gamma-hydroxybutyrate ([3H]GHB), which is specifically bound to sites present in synaptic membrane subcellular fractions of the rat forebrain and the human nucleus accumbens. Elaboration on previous work revealed that acidic pH-induced specific binding of [3H]SUC occurs, and it has been shown to have a biphasic displacement profile distinguishing high-affinity (K(i,SUC) = 9.1 +/- 1.7 microM) and low-affinity (K(i,SUC) = 15 +/- 7 mM) binding. Both high- and low- affinity sites were characterized by the binding of GHB (K(i,GHB) = 3.9 +/- 0.5 microM and K(i,GHB) = 5.0 +/- 2.0 mM) and lactate (LAC; K(i,LAC) = 3.9 +/- 0.5 microM and K(i,LAC) = 7.7 +/- 0.9 mM). Ligands, including the hemiester ethyl-hemi-SUC, and the gap junction inhibitors flufenamate, CBX, and the GHB binding site-selective NCS-382 interacted with the high-affinity site (in microM: K(i,EHS) = 17 +/- 5, K(i,FFA) = 24 +/- 13, K(i,CBX) = 28 +/- 9, K(i,NCS-382) = 0.8 +/- 0.1 microM). Binding of the Na+,K+-ATPase inhibitor ouabain, the proton-coupled monocarboxylate transporter (MCT)-specific alpha-cyano-hydroxycinnamic acid (CHC), and CIT c...Continue Reading

References

Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·N WestergaardA Schousboe
May 1, 1994·Journal of Neurochemistry·N WestergaardA Schousboe
Nov 20, 1998·FEBS Letters·P TrivediX M Guan
Mar 18, 1999·Developmental Genetics·D FrancisM Delmar
May 4, 2000·American Journal of Physiology. Cell Physiology·H WangV Ganapathy
Sep 8, 2001·The Journal of Membrane Biology·V T Schoonderwoert, G J Martens
Jan 10, 2002·Journal of Neuroscience Research·Péter BarabásArne Schousboe
Jan 16, 2002·The Journal of Physiology·Christof J Schwiening, Debbie Willoughby
Feb 13, 2002·Quarterly Reviews of Biophysics·A L Harris
Jun 27, 2002·Pediatric Research·Anne-Marie Van Cappellen Van WalsumRoel Nijland
Nov 26, 2002·Biochemical and Biophysical Research Communications·Katsuhisa InoueVadivel Ganapathy
May 10, 2003·Pflügers Archiv : European journal of physiology·Andrew P Halestrap, David Meredith
Sep 2, 2003·Trends in Neurosciences·Oleg Krishtal
Sep 25, 2003·Physiological Reviews·Mitchell Chesler
Oct 31, 2003·Trends in Neurosciences·Michael V L BennettJuan C Sáez
Mar 16, 2004·The European Journal of Neuroscience·Bálint LasztócziJulianna Kardos
Apr 20, 2004·The Journal of Physiology·Michael J CoadyJean-Yves Lapointe
Sep 14, 2004·The Journal of Biological Chemistry·Sébastien SerresMichel Merle
Jun 1, 2005·Biochimica Et Biophysica Acta·Gina E Sosinsky, Bruce J Nicholson
Aug 16, 2005·Nature·Glenda C HarrisGary Aston-Jones
Oct 8, 2005·Pflügers Archiv : European journal of physiology·Ana M Pajor
Oct 26, 2005·Archives of Disease in Childhood·S L ChowJ V Leonard
May 30, 2006·Neuron·Michael M ScottJoel K Elmquist
May 30, 2006·Neuron·Denis BurdakovAlexei Verkhratsky
Aug 15, 2006·Trends in Neurosciences·Glenda C Harris, Gary Aston-Jones
Oct 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Luis de LeceaRalph DiLeone
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiang-ming ZhaMichael J Welsh
Dec 14, 2006·Neuroreport·Eva SzáricsJulianna Kardos
Feb 15, 2007·Nature Reviews. Neuroscience·Takeshi Sakurai
Mar 16, 2007·Journal of Neurochemistry·Jacqueline Burré, Walter Volknandt
May 16, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Qi Wang, Marilyn E Morris
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rhîannan H WilliamsDenis Burdakov

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Citations

Oct 5, 2011·BMC Neuroscience·Tünde MolnárJulianna Kardos
Apr 6, 2013·Journal of Nanobiotechnology·Gabriella NyitraiJulianna Kardos
Mar 28, 2017·Progress in Neurobiology·Julianna KardosMiklós Palkovits
May 28, 2019·Molecular Neurobiology·Julianna KardosLászló Héja
Jul 25, 2021·Trends in Endocrinology and Metabolism : TEM·Sonia Fernández-VeledoJoan Vendrell

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