PMID: 9191901May 1, 1997Paper

Gamma-irradiation and UV-C light-induced lipid peroxidation: a Fourier transform-infrared absorption spectroscopic study

International Journal of Radiation Biology
R KinderJ M Wessels

Abstract

Fourier transform-infrared spectroscopy of dry, multibilayer films has been used to study gamma-radiation and UV-C light induced lipid peroxidation in 1,2-dilinoleoyl-sn-glycero-3-phosphocholine liposomes. The observed spectral changes were compared with the results obtained from measurement of hydroperoxides, conjugated dienes and to the formation of thiobarbituric acid reactive substances, such as malondialdehyde (MDA) or MDA-like substances. Upon irradiation a decrease in intensity of the asymmetric C - H stretching vibration (va(CH2)) of the isolated cis C = C - H groups (3010 cm-1) was observed. Directly correlated with the decrease of the va(CH2) absorption was a shift of the asymmetric phosphate ester stretching vibration (va(P = O)) towards smaller wavenumbers (1260-->1244 cm-1), indicating that the lipid peroxidation induced molecular alterations in the fatty acid chains influence the packing of the phospholipids in dry multibilayer films. In addition, the formation of a new absorption band at 1693 cm-1 could be detected, the intensity of which was comparable with the formation of thiobarbituric acid reactive substances and, therefore, attributed to the (C = O) stretching of alpha, beta unsaturated aldehydes. Dose-depe...Continue Reading

References

Jan 1, 1979·Journal of Biochemical and Biophysical Methods·D G CameronH H Mantsch
May 21, 1976·Biochimica Et Biophysica Acta·A Petkau, W S Chelack
Jul 22, 1991·Biochimica Et Biophysica Acta·G Stark
Jan 1, 1988·Annals of the New York Academy of Sciences·G Minotti
Oct 17, 1968·European Journal of Biochemistry·W G Niehaus, B Samuelsson
Jan 1, 1984·Methods in Enzymology·T F Slater
Jan 1, 1984·Methods in Enzymology·W A Pryor, L Castle
Aug 1, 1980·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S C PurohitR B Cundall
May 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·B Bose, S N Chatterjee
Apr 1, 1994·Analytical Biochemistry·R H SillsR Mendelsohn

❮ Previous
Next ❯

Citations

Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·Nathalie GaultJean-Louis Lefaix
May 15, 2002·Archives of Pharmacal Research·Hyun Dong JeUy Dong Sohn
Feb 2, 2006·International Journal of Radiation Biology·Nathalie GaultJean-Louis Lefaix
Jul 16, 2003·Radiation Research·Nathalie Gault, Jean-Louis Lefaix
Apr 27, 2010·The Journal of Physical Chemistry. B·Michael C Howland, Atul N Parikh
Dec 5, 2002·Archives of Biochemistry and Biophysics·Anne-Marie MelinGérard Déléris
Nov 11, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Wei JiangBaoshan Xing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Biochemistry, Molecular Biology, and Biophysics : JBMBB : the Official Journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)
B N Pandey, K P Mishra
© 2021 Meta ULC. All rights reserved