Gamma irradiation of aloe-emodin induced structural modification and apoptosis through a ROS- and caspase-dependent mitochondrial pathway in stomach tumor cells

International Journal of Radiation Biology
Eui-Baek ByunEui-Hong Byun

Abstract

The changes in molecular structure and the physiological properties of a gamma-irradiated aloe-emodin were examined. Aloe-emodin was gamma-irradiated at doses ranging from 0 to 150 kGy, and the molecular structure was then analyzed using high-performance liquid chromatography (HPLC). AGS cells were cultured in RPMI medium and treated gamma irradiated aloe-emodin. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptosis efficiency was investigated by cell cycle arrest, cell morphology, and signaling pathway. The structure of new radiolytic peak was identified by the hydrogen-nuclear magnetic resonance (1H NMR). HPLC results showed that gamma irradiation induced new radiolytic peaks that were distinguishable from the aloe-emodin standard, and the area of new peaks was increased as the radiation dose increased. Gamma-irradiated aloe-emodin treatment significantly increased the cytotoxicity in AGS tumor cells. We also found that 150 kGy aloe-emodin increased the expression of Bax, cytosolic cytochrome c, PARP cleavage, and the activation of caspases-8, -9, -3, Bid, and Bcl-2. Treatment of 150 kGy aloe-emodin induced ROS production, DNA fragmentation, alterations of cell...Continue Reading

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Citations

Jul 7, 2020·Chemistry & Biodiversity·Gyeong Han Jeong, Tae Hoon Kim
Feb 18, 2021·Cell Reports·Yuejing JiangYi Zang
Jun 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Henry A AdeolaRashmi Bhardwaj
Jan 4, 2022·Journal of Cancer Research and Clinical Oncology·Dechao JiaoXinwei Han

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