PMID: 8592683Nov 1, 1995Paper

Gamma-irradiation of non-frozen, frozen, and freeze-dried liposomes

Pharmaceutical Research
N J ZuidamD J Crommelin

Abstract

The aim of this work was to investigate the possibilities and limitations of gamma-irradiation as a sterilisation method for nonfrozen, frozen, and freeze-dried liposomes. Liposomes with an average size of 0.2 micron were irradiated with doses up to about 5 x 10(4) Gy in a nitrogen atmosphere. Phospholipids in dipalmitoylphosphatidycholine/dipalmitoylphosphatidylglycerol (DPPC/DPPG) 10/1-liposomes and egg phosphatidylcholine/egg phosphatidylglycerol (EPC/EPG) 10/1-liposomes in 10 mM phosphate buffer (pH 7.4) without trehalose degraded considerably upon gamma-irradiation. Irradiation damage was reduced in the presence of 10% trehalose added as a cryoprotectant, but trehalose reacted with species induced by gamma-irradiation as demonstrated by large decreases in pH. Both pH decrease and oxidative damage of EPC/EPG 10/1-liposomes were strongly dependent on the physical state during irradiation (nonfrozen, frozen or freeze-dried). No changes in liposomal size were found upon gamma-irradiation, and hardly any change was seen in bilayer rigidity. Differences in the gel-to-liquid phase transition of DPPC/DPPG 10/1-liposome dispersions before and after gamma-irradiation were small in the presence of 10% trehalose, but larger in the abs...Continue Reading

Citations

Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·H VitracM Gardès-Albert
Apr 7, 2016·Pharmaceutical Development and Technology·F SakarY Zencir
Apr 30, 2020·Therapeutic Delivery·Kenchappa VanajaHagalavadi Nanjappa Shivakumar
Feb 7, 2021·International Journal of Pharmaceutics·Kouka Luc DelmaGéraldine Piel
Apr 17, 1999·International Journal of Pharmaceutics·G StensrudJ Karlsen
Jan 1, 1997·Free Radical Biology & Medicine·A M SamuniN J Zuidam
May 27, 2015·Chemical Reviews·Bhushan S PattniVladimir P Torchilin

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