PMID: 8960508Nov 1, 1996Paper

Gamma radiation effect on vascular smooth muscle cells in culture

International Journal of Radiation Oncology, Biology, Physics
C GajdusekM R Mayberg

Abstract

Prior work in our laboratory demonstrated that external gamma irradiation administered within 48 h following balloon catheter carotid artery injury in rats produced a marked inhibition of intimal hyperplasia and restenosis. The current study used smooth muscle cells (SMC) in vitro to examine the radiation dose response and to investigate the cellular mechanism by which radiation inhibits SMC proliferation. Quiescent rat aortic SMC in plasma were refed with whole blood serum to stimulate synchronous proliferation and immediately irradiated with single fraction doses of 1.25-20 Gy. Comparison between a micronucleus assay and a clonogenic assay indicated a dose-dependent inhibition of SMC growth, with an ED50 at 2-3 Gy. The micronucleus assay also demonstrated a dose-dependent increase in apparent chromosomal damage at 72 h after irradiation. Inhibition of SMC growth by radiation did not correlate with changes in intracellular or released mitogenic activity. Furthermore, there was no evidence of apoptosis in irradiated SMC up to 96 h after treatment. Radiation likely inhibits SMC proliferation after arterial injury by a dose-dependent mechanism of lethal and/or sublethal cellular injury leading to clonogenic cell death.

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Citations

May 31, 2001·International Journal of Radiation Oncology, Biology, Physics·M KollumR Waksman
May 31, 2001·International Journal of Radiation Oncology, Biology, Physics·N A ScottK K Griendling
Apr 8, 1998·International Journal of Radiation Oncology, Biology, Physics·P RubinR M Green
Mar 7, 2000·Journal of the American College of Surgeons·K A IlligR M Green
Jun 16, 2005·Kidney International·Seung-Jung KimAlfred K Cheung
Apr 16, 1998·Journal of Vascular and Interventional Radiology : JVIR·D A Diamond, T M Vesely
Nov 7, 1998·Current Problems in Cardiology·W H FrishmanM Weiss
Jun 1, 2000·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·A T UlusM Bayazit
May 31, 2001·Journal of Cellular Physiology·C GajdusekM Mayberg
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Yong-Jian Geng, Peter Libby
Nov 1, 2018·Cellular and Molecular Life Sciences : CMLS·Bjorn BaseletAn Aerts
Jan 8, 2000·Neurosurgery·M M O'Connor, M R Mayberg

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