Gamma radiation induced mutagenesis in Aspergillus niger to enhance its microbial fermentation activity for industrial enzyme production.

Molecular Biology Reports
M Siddique AwanM I Rajoka

Abstract

α- and β-Galactosidases find application in food processing, health and nutrition. Aspergillus niger is one of the potent producer of these enzymes and was genotypically improved using gamma-ray induced mutagenesis. The mutant-derivative produced two-fold higher α- and β-galactosidases. For testing genetic variability and its relationship with phenotypic properties of the two organisms, DNA samples of the mutant and parental strains of A. niger were amplified with 28 deca-nucleotide synthetic primers. RAPD analysis showed significantly different pattern between parental and mutant cultures. The mutant derivative yielded homogeneous while parental strain formed heterogeneous amplification patterns. Seven primers identified 42.9% polymorphism in the amplification products, indicating that these primers determined some genetic variability between the two strains. Thus RAPD was found to be an efficient technique to determine genetic variability in the mutant and wild organisms. Both wild and mutant strains were analyzed for their potential to produce galactosidases. Comparison of different carbon sources on enzyme yield revealed that wheat bran is significant (P < 0.01) effective producer and economical source followed by rice bran...Continue Reading

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Citations

Sep 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad AzeemKhizar Hayyat
Jan 13, 2015·Mycologia·Rodrigo A OlarteIgnazio Carbone
Sep 18, 2013·Applied Biochemistry and Biotechnology·Wei LiZhiqun Liang
May 10, 2011·Molecular Biology Reports·Shafiquzzaman SiddiqueeMd Mainul Hasan
Nov 28, 2013·Preparative Biochemistry & Biotechnology·Weibing ZhangZhigang An
Aug 15, 2015·Clinical and Experimental Vaccine Research·Ho Seong Seo

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