PMID: 8600354Feb 1, 1996Paper

Gamma-radiation-induced mutation spectrum in the episomal lacI gene of Escherichia coli under oxic conditions

Mutation Research
C A WijkerJ Retèl

Abstract

In this study we have determined the mutation spectrum in the complete episomal lacI gene of Escherichia coli induced by gamma-radiation under oxic conditions. Mutants were generated by 60Co gamma-irradiation of an E. coli culture of stationary cells in LB medium, under continuous flushing with oxygen. Oligonucleotide probe analysis showed that 14% of the gamma-ray-induced mutations were located at the lacI gene hot spot at position 620-632, which is characterized by a triple repeat of the 5'-TGGC-3' sequence. Previously it was shown that about 70% of the spontaneous mutations were located at this site due to the loss or the addition of a TGGC sequence. The non-hot spot mutations were further characterized by automated sequence analysis. The results show that base pair (bp) substitutions were the main type of gamma-ray-induced mutations. Although all types of bp substitutions were observed, 74% of the bp substitutions involved C/G base pairs. C/G --> T/A and C/G --> A/T substitutions were predominant, both accounting for 35% of all bp substitutions, whereas A/T --> C/G substitutions were only seldomly observed (3%). A relatively large amount of -1 bp deletions (15% of all mutations) was detected in the gamma-ray-induced mutatio...Continue Reading

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Citations

May 7, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D S KimS-Y Kang
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Baross-FrancisF R Jirik
Sep 6, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kyung Jun LeeDong Sub Kim
Feb 13, 2010·International Journal of Radiation Biology·Ryouhei YoshiharaIssay Narumi

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