PMID: 6972362May 1, 1981Paper

Gamma radiation inhibits the appearance of induced ornithine decarboxylase activity in Chinese hamster cells

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
E Ben-HurE Riklis

Abstract

Ornithine decarboxylase activity of Chinese hamster cells (ODC, EC 4.1.1.17) can be induced in plateau phase by change of medium. Exposure of the cells to gamma radiation before induction reduces the amount of ODC activity induced. The dose-response curve is exponential with a D0 of 106 krad. Exposure of BUdR-substituted cells is more effective in reducing ODC induction at high doses, with a D0 of 38 krad. Cells can recover from the reduction incurred by 74 krad if enzyme induction is delayed for 2 hours after exposure. Treatment of the cells with psoralen-plus-light completely inhibits RNA synthesis without affecting protein synthesis (Heimer, Ben-Hur and Riklis 1977, 1978). Using this procedure it is shown that the effect of gamma radiation on inducible ODC activity is due not only to DNA damage by also involves a post-transcriptional effect. This conclusion is supported by employing a heat shock to inhibit protein synthesis prior to gamma-irradiation of log-phase cells. In such cells the increased activity of ODC upon transfer to 37 degrees C is due primarily to enzyme synthesis using pre-existing RNA species during the first few hours. A low concentration of actinomycin D, which inhibits rRNA synthesis, applied during the r...Continue Reading

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·U Bachrach
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W Murphy, G Attardi
Oct 5, 1973·Journal of Molecular Biology·R P Perry, D E Kelley
Dec 1, 1969·Archives of Biochemistry and Biophysics·N Fausto, J L Van Lancker
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Sep 1, 1966·Journal of Molecular Biology·W K Roberts, J F Newman
Apr 1, 1965·Archives of Biochemistry and Biophysics·T UCHIYAMAJ L VANLANCKER

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