Gamma synuclein is a novel nicotine responsive protein in oral cancer malignancy

Cancer Cell International
Chia-Chen HsuYi-Shing Shieh

Abstract

The mechanisms of neuronal protein γ-synuclein (SNCG) in the malignancy of oral squamous cell carcinoma (OSCC) are not clear. This study tested the hypothesis that SNCG is involved in nicotine-induced malignant behaviors of OSCC. The effect of nicotine on SNCG expression and epithelial-to-mesenchymal transition (EMT) markers were examined. Short hairpin RNA (shRNA) and an antagonist specific for α7-nicotine acetylcholine receptors (α7-nAChRs) were used to examine the role of α7-nAChRs in mediating the effects of nicotine. Knockdown of SNCG in nicotine-treated cells was performed to investigate the role of SNCG in cancer malignancy. The in vivo effect of nicotine was examined using a nude mouse xenotransplantation model. Nicotine increased SNCG expression in a time- and dose-dependent manner. Nicotine treatment also increased E-cadherin and ZO-1 and decreased fibronectin and vimentin expression. After specific knockdown of α7-nAChRs and inhibition of the PI3/AKT signal, the effect of nicotine on SNCG expression was attenuated. Silencing of SNCG abolished nicotine-induced invasion and migration of OSCC cells. The xenotransplantation model revealed that nicotine augmented tumor growth and SNCG expression. Nicotine upregulated SNCG...Continue Reading

References

Sep 16, 1998·Human Genetics·C LavedanM H Polymeropoulos
Jul 12, 2003·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Wen-Liang LoRichard Che-Shoa Chang
Jun 29, 2004·Cancer·Zhongkui LiPaul J Chiao
Jun 15, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mushfika AhmadOmar M A El-Agnaf
Nov 18, 2008·Biochimica Et Biophysica Acta·Dong-Pyo HongVladimir N Uversky
Mar 30, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chih-Hsiung WuYuan-Soon Ho
Nov 15, 2012·Journal of Neuroscience Research·Andrei Surguchov
Dec 6, 2014·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jiyu Zhao, Nianzeng Xing
Dec 15, 2015·Journal of Dental Research·J C ChengY S Shieh
Mar 17, 2016·Journal of Oncology·Yue Zhao
May 12, 2017·Frontiers in Oncology·Marco CorazzariMauro Piacentini
Jun 4, 2018·Neurobiology of Disease·Sudhakar R SubramaniamMarie-Francoise Chesselet

❮ Previous
Next ❯

Citations

Aug 31, 2020·Cancer Cell International·Chia-Chen HsuYi-Shing Shieh

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
Transfection
xenografts
xenograft
protein folding

Software Mentioned

GraphPad Prism
Image J

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.