PMID: 32521859Jun 12, 2020Paper

Gammacerane suppresses the growth of human endometrial carcinoma cells via induction of apoptosis, cell cycle arrest, inhibition of cell migration, invasion and STAT3 signaling pathway

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Chenming WangXiuying Yue

Abstract

This study was conducted to assess the anticancer role of gammacerane against human endometrial cancer. The human RL-95 cell line (endometrial cancer) and SV40 (normal endometrial cells) were used in this study. The MTT-based estimation of cell proliferation assay along with the colony formation assay were used for assessing the cell viability. Acridine orange (AO)/Ethidium bromide (EB) staining followed by fluorescent microscopy was performed for estimation of cell apoptosis. Flow cytometry was used to assess the cell cycle phase distribution of cancer cells. Cell migration and invasion were estimated using wound healing and transwell assay, respectively. Western blotting was used for protein expression studies. The cell proliferation assay revealed that gammacerane treatment led to loss of viability of RL-95 cancer cells in a concentration-dependent manner. However, the antiproliferative effects were comparatively less prominent when gammacerane was used against the SV40 normal endometrial cells. AO/EB staining of cancer cells showed that gammacerane is active in inducing apoptosis in RL-95 cells and apoptotic induction effects were more evident at higher concentrations of the molecule. Flow cytometric analysis with Annexin V...Continue Reading

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