PMID: 9175912Jun 1, 1997Paper

Gammalinolenic acid and dihomogammalinolenic acid suppress the CD3-mediated signal transduction pathway in human T cells

Clinical Immunology and Immunopathology
D VassilopoulosG C Tsokos

Abstract

Gammalinolenic acid (GLA; 18:3n6) and dihomogammalinolenic acid (DGLA; 20:3n6) suppress lymphocyte activation, and GLA administration reduces joint swelling and tenderness in rheumatoid arthritis patients with active synovitis. In an effort to dissect the mechanisms whereby GLA, DGLA, and other fatty acids influence lymphocyte function, we examined their effects on anti-CD3 monoclonal antibody (mAb)-mediated early signaling events in human T cells. Peripheral blood mononuclear cells from healthy individuals were incubated overnight at 37 degrees C with or without 10 microg/ml fatty acid and then loaded with the calcium binding fluorescent dye indo-1. Fatty acids did not affect the efficiency of indo-1 loading, and they did not alter cell surface membrane expression of the CD3 molecule. Anti-CD3 mAb (G19-4)-induced intracellular calcium [(Ca2+)i] changes were monitored by flow cytometry in negatively selected human T cells. The ratio of violet to blue fluorescence, which is proportional to (Ca2+)i, was measured over time. Cells enriched with GLA and DGLA but not cells enriched with eicosapentaenoic acid (20:5n3) displayed a significant reduction in anti-CD3 mAb-induced early and late (Ca2+)i responses. T cells loaded with GLA, D...Continue Reading

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Citations

Mar 30, 1999·The Proceedings of the Nutrition Society·P C Calder
Mar 30, 1999·The Proceedings of the Nutrition Society·G FernandesC A Jolly
Sep 18, 2007·Applied and Environmental Microbiology·Hisashi YazawaHiroshi Uemura
Oct 19, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Barbara C OlendzkiRobert B Zurier
Mar 4, 2008·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Karolien H KokkeJohn G Lawrenson
Jun 27, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Yung-Chih ChenMai-Szu Wu
Jun 15, 2006·Journal of Dairy Science·K KimuraJ P Goff
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·K MayerF Grimminger
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·P Yaqoob
Sep 10, 1998·The Journal of Nutrition·Y Y Fan, R S Chapkin
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R K FurseR B Zurier
Dec 1, 2001·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S KakuK Yamada

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