PMID: 3769920Oct 1, 1986Paper

Ganglioside biosynthesis in rat liver. Characterization of three sialyltransferases

European Journal of Biochemistry
K Busam, K Decker

Abstract

Three sialyltransferase activities involved in ganglioside biosynthesis were studied in Golgi-enriched preparations of rat liver: the formation of GM3, GD3 and GD1a. The conditions for the quantitative assays of these enzymatic reactions were standardized and optimized, with Triton X-100 being used as detergent. The apparent Km values of each sialyltransferase for N-acetyl-2-(5'-cytidylyl)neuraminic acid (1.5 mM with GM3 synthase, 0.2 mM with GD3 synthase, and 0.5 mM with GD1a synthase) and the respective glycolipid substrates (0.08 mM for lactosylceramide, 0.1 mM for GM3, and 0.5 mM for GM1) were determined. Competition experiments showed that the three sialyltransferase activities are three individual catalytic entities. Moreover, evidence was found that product inhibition may play a role in the regulation of the activity of sialyltransferases.

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Citations

Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G K Ostrander, E H Holmes
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G PohlentzK Sandhoff
Nov 6, 1989·European Journal of Biochemistry·R Schüz-HenningerK Decker
Aug 1, 1998·Biological Chemistry·M Anić, M Mesarić
Jan 13, 2000·Journal of Basic and Clinical Physiology and Pharmacology·E OzkökB Güvener
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·J F PoschetV Deretic
Apr 1, 1988·Archives of Biochemistry and Biophysics·R Schüz-HenningerK Decker
Jul 19, 1993·FEBS Letters·Y H HwangW Reutter
Dec 1, 1991·Biochemical Genetics·A M Novikov, T N Seyfried
Mar 28, 2017·Frontiers in Molecular Neuroscience·Marcus O W GrimmTobias Hartmann
Aug 15, 2001·American Journal of Physiology. Cell Physiology·G ChandyT E Machen
Jun 1, 1992·The American Journal of Physiology·C B Zeller, R B Marchase
Feb 1, 1992·Trends in Cell Biology·J Barasch, Q Al-Awqati
Aug 31, 1990·Biochemical and Biophysical Research Communications·M Mesarić, K Decker
Jan 31, 1991·Biochemical and Biophysical Research Communications·J B FishmanM D Ullman

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