Ganglioside composition of differentiated Caco-2 cells resembles human colostrum and neonatal rat intestine

The British Journal of Nutrition
Kareena L SchnablM Thomas Clandinin

Abstract

Gangliosides are glycosphingolipids found in cell membranes and human milk with important roles in cell proliferation, differentiation, growth, adhesion, migration, signalling and apoptosis. Similar changes in ganglioside composition occur during embryonic development, lactation and cancer cell differentiation. It is not known, however, whether ganglioside compositional changes that occur in differentiating colon cancer cells reflect changes that occur during intestinal development. The Caco-2 cell line is commonly used to study physiological and pathophysiological processes in the small intestine and colon. Therefore, to examine this question, undifferentiated and differentiated Caco-2 cells were grown and total lipid was extracted from cell supernatant fractions using the Folch method. The upper aqueous phase containing gangliosides was collected and purified. Total gangliosides were measured as ganglioside-bound N-acetyl neuraminic acid, while individual ganglioside content was quantified via a colorimetric assay for sialic acid and scanning densitometry. The total ganglioside content of differentiated Caco-2 cells was 2.5 times higher compared with undifferentiated cells. Differentiated Caco-2 cells had significantly more (...Continue Reading

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Citations

May 7, 2010·International Journal of Food Microbiology·Avrelija Cencic, Tomaz Langerholc
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Jan 27, 2011·Angewandte Chemie·Thomas HaselhorstMark von Itzstein

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