Ganglioside GD1a suppresses TNFalpha expression via Pkn1 at the transcriptional level in mouse osteosarcoma-derived FBJ cells

Biochemical and Biophysical Research Communications
Li WangTatsuya Yamagata

Abstract

Ganglioside GD1a has been reported to suppress metastasis [S. Hyuga, S. Yamagata, Y. Takatsu, M. Hyuga, H. Nakanishi, K. Furukawa, T. Yamagata, Suppression of FBJ-LL cell adhesion to vitronectin by ganglioside GD1a and loss of metastatic capacity, International J. Cancer. 83 (1999) 685-691.] and MMP-9 production in mouse osteosarcoma FBJ cells [D. Hu, Z. Man, P. Wang, X. Tan, X. Wang, S. Takaku, S. Hyuga, T. Sato, X. Yao, S. Yamagata, T. Yamagata, Ganglioside GD1a negatively regulates MMP9 expression in mouse FBJ cell lines at the transcriptional level, Connect. Tissue Res. 48 (2007) 198-205.]. In the present study, TNFalpha increased cell motility and MMP-9 and TNFalpha expression at the transcriptional level. TNFalpha expression was found to be inversely proportional to GD1a content in the FBJ-cell variants. The addition of exogenous GD1a to FBJ-LL cells suppressed TNFalpha expression, and treatment of FBJ-S1 cells with D-PDMP (glucosylceramide synthesis inhibitor) led to an increase in TNFalpha, indicating that TNFalpha is negatively regulated by GD1a in FBJ cells. SiRNA of Pkn1, a Rho-GTPase effecter protein kinase, suppressed TNFalpha levels as well as Pkn1 expression, suggesting that Pkn1 is involved in TNFalpha signaling...Continue Reading

References

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Sep 1, 2006·Biochemical and Biophysical Research Communications·Dan HuTatsuya Yamagata

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Citations

Feb 11, 2016·The Journal of Reproduction and Development·Jin-Woo KimDeog-Bon Koo
Jul 1, 2010·Journal of Cellular Biochemistry·Ting CaoTatsuya Yamagata
Oct 30, 2013·The FEBS Journal·Giovanni D'AngeloDomenico Russo
Nov 26, 2019·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Pinelopi ArvanitiYves Renaudineau
Oct 26, 2010·Progress in Lipid Research·Gauri A Patwardhan, Yong-Yu Liu

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