Ganglioside GD3 expression on target cells can modulate NK cell cytotoxicity via siglec-7-dependent and -independent mechanisms

European Journal of Immunology
Gavin NicollPaul R Crocker

Abstract

Siglec-7 is a sialic acid binding receptor with inhibitory potential, expressed on human NK cells and monocytes. It has an unusual binding preference for alpha2,8-linked disialic acids, such as those displayed by ganglioside GD3. Here we have investigated whether siglec-7-GD3 interactions are able to modulate NK cell cytotoxicity. Using synthetic polyacrylamide glycoprobes, siglec-7 was found to be masked at the NK cell surface but it could be unmasked by sialidase treatment of NK cells. GD3 synthase-transfected P815 target cells expressed high levels of GD3 and bound strongly to recombinant siglec-7-Fc protein. Surprisingly, GD3 synthase-transfected P815 cells were killed more effectively by untreated cells in a siglec-7-independent manner. However, following sialidase treatment of NK cells, a siglec-7-dependent inhibition of killing was observed. These findings have important implications for NK cell cytotoxicity against tumor cells like melanoma that express high levels of GD3 ganglioside.

Citations

Jan 18, 2007·Histochemistry and Cell Biology·Elena I KovalenkoNicolai Bovin
Jun 16, 2005·Current Opinion in Pharmacology·Paul R Crocker
Dec 31, 2003·Trends in Immunology·Timo K van den BergGary W Litman
Feb 21, 2008·Reproductive Biomedicine Online·Wayne M Yokoyama, Joan K Riley
Apr 21, 2005·Nature Reviews. Immunology·Vinay Kumar, Megan E McNerney
Mar 24, 2007·Nature Reviews. Immunology·Paul R CrockerAjit Varki
Mar 18, 2005·Annual Review of Immunology·Lewis L Lanier
Aug 9, 2007·BMC Immunology·Jeannette M MoebiusChristoph Piechaczek
Feb 27, 2014·The Journal of Clinical Investigation·Camilla JandusStephan von Gunten
Mar 27, 2007·Future Oncology·Mepur H RavindranathDonald L Morton
Sep 23, 2014·Nature Reviews. Immunology·Matthew S MacauleyJames C Paulson
Jan 10, 2012·Annual Review of Immunology·Shiv PillaiHamid Mattoo
Dec 3, 2013·Nature Chemical Biology·Jason E HudakCarolyn R Bertozzi
Dec 19, 2013·Nature Chemical Biology·Matthew S Macauley, James C Paulson
Aug 11, 2015·Chemistry & Biology·Brian Belardi, Carolyn R Bertozzi
Jul 17, 2012·Taiwanese Journal of Obstetrics & Gynecology·Wen-Chi LeePeng-Hui Wang
Aug 12, 2009·Biochimica Et Biophysica Acta·Shuguang Bi, Linda G Baum
Feb 25, 2009·Human Immunology·Annika ErbacherIngo Müller
Feb 3, 2004·Journal of Cellular and Molecular Medicine·R BiassoniN Dimasi
Apr 25, 2012·Annals of the New York Academy of Sciences·Gabriel A RabinovichBrian A Cobb
Feb 1, 2012·Annals of the New York Academy of Sciences·James C PaulsonNorihito Kawasaki
May 16, 2012·European Journal of Immunology·Friederike GiesekeIngo Müller
Nov 24, 2004·Current Opinion in Chemical Biology·Brian E Collins, James C Paulson
Dec 8, 2004·International Journal for Parasitology·Daniel S KorbelEleanor M Riley
Jun 11, 2011·Biochemical Pharmacology·Camilla JandusStephan von Gunten
Dec 30, 2014·European Journal of Immunology·Michael BrusilovskyAngel Porgador
Jul 16, 2014·Biochimica Et Biophysica Acta·Christian BüllGosse J Adema
Jan 28, 2014·Cytotechnology·Toru ShojiShinji Iijima
Nov 21, 2007·Journal of Molecular Biology·Marina A ZhuravlevaPeter D Sun
Jan 8, 2011·Analytical Biochemistry·Diana M E OttoPaul R Crocker
Aug 24, 2006·Biochimica Et Biophysica Acta·Bruno SéguiThierry Levade
Dec 10, 2014·Cellular and Molecular Life Sciences : CMLS·Kayluz Frias BoliganStephan von Gunten
Jun 7, 2014·Frontiers in Oncology·Nora Berois, Eduardo Osinaga
May 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shih-Chi YehChi-Huey Wong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.