Ganglioside GD3 traffics from the trans-Golgi network to plasma membrane by a Rab11-independent and brefeldin A-insensitive exocytic pathway.

The Journal of Biological Chemistry
Pilar M CrespoJose L Daniotti

Abstract

Gangliosides, complex glycosphingolipids containing sialic acids, have been found to reside in glycosphingolipid-enriched microdomains (GEM) at the plasma membrane. They are synthesized in the lumen of the Golgi complex and appear unable to translocate from the lumenal toward the cytosolic surface of Golgi membrane to access the monomeric lipid transport. As a consequence, they can only leave the Golgi complex via the lumenal surface of transport vesicles. In this work we analyzed the exocytic transport of the disialo ganglioside GD3 from trans-Golgi network (TGN) to plasma membrane in CHO-K1 cells by immunodetection of endogenously synthesized GD3. We found that ganglioside GD3, unlike another luminal membrane-bounded lipid (glycosylphosphatidylinositol-anchored protein), did not partition into GEM domains in the Golgi complex and trafficked from TGN to plasma membrane by a brefeldin A-insensitive exocytic pathway. Moreover, a dominant negative form of Rab11, which prevents exit of vesicular stomatitis virus glycoprotein from the Golgi complex, did not influence the capacity of GD3 to reach the cell surface. Our results strongly support the notion that most ganglioside GD3 traffics from the TGN to the plasma membrane by a non-...Continue Reading

References

Aug 23, 1988·Biochemistry·K Simons, G van Meer
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E WarnockJ U Baenziger
Nov 1, 1996·The Journal of Cell Biology·O UllrichR G Parton
Jun 5, 1997·Nature·K Simons, E Ikonen
Feb 1, 2000·Trends in Cell Biology·C L Jackson, J E Casanova
Jun 22, 2000·The Journal of Biological Chemistry·R LiS Ladisch
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Feb 15, 2001·Nature Cell Biology·P KellerK Simons
Mar 10, 2001·Biochemical and Biophysical Research Communications·A L ZiulkoskiF C Guma
May 2, 2001·The Journal of Cell Biology·B J NicholsJ Lippincott-Schwartz
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Jul 17, 2001·Current Opinion in Cell Biology·M S Robinson, J S Bonifacino
Aug 30, 2001·Cell·F GalbiatiM P Lisanti
Jan 30, 2002·Cellular and Molecular Life Sciences : CMLS·S Chatterjee, S Mayor
May 16, 2002·The Journal of Biological Chemistry·Gerrit van Meer, Quirine Lisman
Oct 2, 2002·FEBS Letters·Tounsia Aït Slimane, Dick Hoekstra
Dec 24, 2002·Trends in Cell Biology·David L Marks, Richard E Pagano
Jun 14, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard E Pagano
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Gerrit J K Praefcke, Harvey T McMahon
Jun 9, 2004·European Journal of Biochemistry·Adolfo R ZuritaJosé L Daniotti

❮ Previous
Next ❯

Citations

Jul 20, 2011·The Journal of Biological Chemistry·Aldo A VilcaesJose L Daniotti
Dec 7, 2013·PloS One·Blaine BiselFrancesco Saverio Pavone
Jun 24, 2011·IUBMB Life·Jose Luis Daniotti, Ramiro Iglesias-Bartolomé
Sep 26, 2006·Biochimica Et Biophysica Acta·Anthony H Futerman
Jul 20, 2010·The Journal of Biological Chemistry·Pilar M CrespoJosé L Daniotti
Feb 6, 2017·Journal of Immunology Research·Julien FleurenceStéphane Birklé
Feb 9, 2020·International Journal of Molecular Sciences·Aldo A VilcaesJose L Daniotti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Biology of Lipids

Lipids are essential in many biological functions, including membrane structure, energy storage, and cell signaling. Find the latest research on the cell biology of lipids here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

EMBO Reports
A H Futerman, Yusuf A Hannun
Nature Reviews. Molecular Cell Biology
Maria Antonietta De Matteis, Alberto Luini
Seminars in Cell & Developmental Biology
Dan J Sillence, Frances M Platt
© 2021 Meta ULC. All rights reserved