Ganglioside GM1 Contributes to the State of Insulin Resistance in Senescent Human Arterial Endothelial Cells.

The Journal of Biological Chemistry
Norihiko SasakiMasashi Toyoda

Abstract

Vascular endothelial cells (ECs) play central roles in physiologically important functions of blood vessels and contribute to the maintenance of vascular integrity. Therefore, it is considered that the impairment of EC functions leads to the development of vascular diseases. However, the molecular mechanisms of the EC dysfunctions that accompany senescence and aging have not yet been clarified. The carbohydrate antigens carried by glycoconjugates (e.g. glycoproteins, glycosphingolipids, and proteoglycans) mainly present on the cell surface serve not only as marker molecules but also as functional molecules. In this study, we have investigated the abundance and functional roles of glycosphingolipids in human ECs during senescence and aging. Among glycosphingolipids, ganglioside GM1 was highly expressed in abundance on the surface of replicatively and prematurely senescent ECs and also of ECs derived from an elderly subject. Insulin signaling, which regulates important functions of ECs, is impaired in senescent and aged ECs. Actually, by down-regulating GM1 on senescent ECs and overloading exogenous GM1 onto non-senescent ECs, we showed that an increased abundance of GM1 functionally contributes to the impairment of insulin signa...Continue Reading

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Citations

Oct 30, 2018·Journal of Alzheimer's Disease : JAD·Andy MadridReid S Alisch
Jan 8, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jelske N van der VeenRené L Jacobs
Apr 20, 2017·Stem Cell Research & Therapy·Norihiko SasakiMasashi Toyoda
Apr 25, 2019·International Journal of Molecular Sciences·Norihiko SasakiMasashi Toyoda
Dec 22, 2019·International Journal of Molecular Sciences·Norihiko Sasaki, Masashi Toyoda
Feb 15, 2021·Cancer Immunology, Immunotherapy : CII·Reem SalehRanad Shaheen
Jul 4, 2021·Biochemical and Biophysical Research Communications·Mira SuzukiTadashi Yamashita
Jan 7, 2020·Current Medicinal Chemistry·Konstantin Andreev
Sep 3, 2021·The Journal of Physical Chemistry. B·Ji Hun WiSo Yeon Kim

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Methods Mentioned

BETA
FACS
immunoprecipitations
dot blot
PCR
flow cytometry
immunoprecipitation

Software Mentioned

ImageJ
Excel

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