PMID: 6413653Nov 1, 1983Paper

Ganglioside-induced neuritogenesis: verification that gangliosides are the active agents, and comparison of molecular species

Journal of Neurochemistry
M C ByrneJ R Sclafani

Abstract

Gangliosides were previously reported to induce neuritogenesis in primary neuronal cultures and in some neurally derived cell lines. Because isolated gangliosides usually contain variable quantities of peptides, we investigated the possibility that neurite-stimulating activity could be caused by these contaminants. Ganglioside preparations from bovine brain and other sources were subjected to a three-step purification procedure that eliminated at least 95% of the contaminating peptides. These purified preparations retained their capacity to induce extensive neurite growth in neuro-2A murine neuroblastoma. Proteolytic digestion and a number of additional procedures were used to reduce residual contamination further without loss of activity. Several crude ganglioside samples had negative effects on neurite development until freed of their inhibitory factors, which were derived from the tissue and/or introduced during laboratory operations. This was particularly evident for bovine white matter gangliosides whose activity increased in proportion to peptide removal. When carefully purified, virtually all of 11 different gangliosides tested were highly active, with the possible exception of GM4, which demonstrated only moderate activ...Continue Reading

References

Jan 1, 1979·Journal of Supramolecular Structure·J I Morgan, W Seifert
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Oct 1, 1973·Journal of Neurochemistry·R W LedeenL F Eng
Jul 1, 1974·Archives of Biochemistry and Biophysics·S De BernardoS Udenfriend
May 1, 1972·Analytical Biochemistry·M B Lees, S Paxman
Jun 1, 1970·Gut·M I JaysonW J Harrison
Apr 1, 1966·Journal of Neurochemistry·W T Norton, L A Autilio
Jan 1, 1980·Journal of Neurochemistry·G P Harper, H Thoenen
Jan 1, 1980·Journal of Virology·P S WuY A Isaacson
Jan 1, 1980·Current Topics in Developmental Biology·S Varon, R Adler
Jan 1, 1982·Developmental Neuroscience·A LeonG Toffano
Jul 1, 1983·Experimental Neurology·M C Byrne, R W Ledeen
Oct 1, 1983·Journal of Neurochemistry·J I MorganW Seifert
Apr 1, 1982·Journal of Neuroimmunology·O S Jørgensen, W Dimpfel
Mar 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K RadsakH Wiegandt
Mar 1, 1981·Stroke; a Journal of Cerebral Circulation·E Siemkowicz, A J Hansen
Oct 30, 1981·Science·F J RoisenG Yorke
Oct 1, 1980·Journal of Neurochemistry·G ToffanoG Tettamanti
Feb 1, 1981·Journal of Neurochemistry·F B CochranR W Ledeen
Jan 1, 1959·Biochimica Et Biophysica Acta·G V MARINETTIE STOTZ
Sep 1, 1963·Journal of Neurochemistry·L SVENNERHOLM
Dec 1, 1964·Journal of Neurochemistry·M MATSUMOTOJ FOLCH-PI

❮ Previous
Next ❯

Citations

Aug 10, 1999·Journal of Neuroscience Research·D Kozireski-ChubackR W Ledeen
Jan 1, 1990·Annals of Neurology·R W LedeenA Cervone
Feb 1, 1992·Journal of Neuroscience Research·K NakamuraR W Ledeen
Jul 1, 1995·Journal of Neuroscience Research·H E SaqrA J Yates
Dec 1, 1986·Neurochemical Pathology·A J Yates
Feb 4, 2010·Molecular Neurobiology·Marco PiccininiAlessandro Prinetti
Sep 1, 1988·Analytical Biochemistry·G S Wu, R Ledeen
Sep 25, 1989·Biochimica Et Biophysica Acta·L SvennerholmB M Rynmark
Jan 1, 1987·Neurochemistry International·M S WilsonA J Yates
Apr 1, 1992·Neurochemistry International·H RahmannS Esders
Jun 24, 1987·Biochimica Et Biophysica Acta·W Curatolo
Jan 1, 1988·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M S CannellaR W Ledeen
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R E Baker, D G Janzen
Mar 30, 2005·Journal of Pediatric Gastroenterology and Nutrition·Eek Joong ParkM Thomas Clandinin
Jul 21, 1998·Annals of the New York Academy of Sciences·A J Yates, A Rampersaud
Jul 21, 1998·Annals of the New York Academy of Sciences·R W LedeenY Fang
Apr 1, 1991·Molecular and Chemical Neuropathology·Y Qi, Q M Xue
Nov 16, 2010·Neurochemical Research·Robert W Ledeen, Gusheng Wu
Jan 6, 2011·Neurochemical Research·Alessandro PrinettiSandro Sonnino
Aug 1, 1989·Pharmacology, Biochemistry, and Behavior·J Mathew, W R Klemm
Oct 15, 1993·Journal of Immunological Methods·F ChabraouiC Caudie
Jan 1, 1990·Alcohol·W R Klemm
May 27, 2015·Nutrients·Kate PalmanoPaul McJarrow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
M S CannellaR W Ledeen
© 2021 Meta ULC. All rights reserved