PMID: 8595950Mar 1, 1996Paper

Gangliosides of migrating and nonmigrating corneal epithelium in organ and cell culture

Investigative Ophthalmology & Visual Science
Z YangN Panjwani

Abstract

To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithelial cell proliferation. [3H]-galactose-labeled and unlabeled glycolipids of migrating and nonmigrating rabbit corneal epithelium in cell and/or in organ culture were chromatographed on DEAE Sephadex to isolate gangliosides. The gangliosides eluted from the ion-exchange column were further characterized by thin-layer chromatography (TLC), glycosidase digestions, and TLC-immunostain analysis. A [3H]-thymidine incorporation assay was used to determine the effect of exogenous gangliosides on corneal epithelium cell proliferation. Upon TLC of the acidic fraction eluted from the DEAE column, only two radiolabeled glycolipids (GL1 and GL2), migrating as a doublet, were detected. Regardless of whether the epithelia were prepared by cell culture or organ culture, both GL1 and GL2 were present in a significantly higher amount in migrating compared to nonmigrating epithelia. Further characterization of GL1 and GL2 identified them a...Continue Reading

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