Ganoderma applanatum secondary metabolites induced apoptosis through different pathways: In vivo and in vitro anticancer studies

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Waill A ElkhateebAhmed E Abdel Moneim

Abstract

Ganoderma applanatum is a widely distributed saprobic or parasitic mushroom, it was found at the bases of decaying logs in Hakozaki Higashi-ku Fukuoka-shi. Japan. The mushroom was extracted with 80% methanol, and LC-HRMS analysis was conducted to illustrate the bioactive ingredients. The cytotoxicity of the total metabolite extract was evaluated against human colon cancer cell line (Caco-2) which showed IC50 value of 160 ± 4.08 μg/ml. G. applanatum methanolic extract caused different morphological alterations and increased glutathione level in the treated cells. Interestingly, G. applanatum increased Bax/Bcl-2 ratio significantly (P ˂ 0.05) at concentrations of 80 and 160 μg/ml on Caco-2 undergoing apoptotic p53-independent pathway with lake expression of p53 protein and up-regulated Cas-3 mRNA. The in vivo study on solid Ehrlich tumor (SEC) revealed a decrease in the volume of the developed tumor mass after five days of G. applanatum (200 μg/ml) treatment. The apoptotic p53-dependant pathway was confirmed by mRNA Bax/Bcl-2 increased ratio in addition to p53 and Cas-3 up-regulation. In conclusion, G. applanatum could exert apoptotic antitumor properties in Caco-2 by p53-independent pathway and p53-dependant in SEC. The findings...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Feb 24, 2004·Journal of Medicinal Food·Noriko KodamaHiroaki Nanba
Oct 15, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Xiang-jian LuoYa Cao
May 1, 2008·Current Protocols in Toxicology·William S StokesMichael Paris
Apr 2, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cui-Cui WangXiu-Kun Lin
Jul 27, 2011·Environmental Toxicology and Pharmacology·Jie-Qiong MaYun-Zhi Sun
Jan 27, 2012·Journal of Natural Products·Donald B StierleJesse Nichols
May 15, 2012·3 Biotech·Seema Patel, Arun Goyal
Oct 16, 2012·The International Journal of Biochemistry & Cell Biology·Thibaud T RenaultStéphen Manon
Nov 6, 2012·Ageing Research Reviews·Venus SosaMatilde E LLeonart
Jul 25, 2014·European Journal of Pharmacology·Shaloam Dasari, Paul Bernard Tchounwou
Aug 13, 2014·BioMed Research International·Monika Osińska-JaroszukMartyna Kandefer-Szerszeń
Dec 19, 2014·Journal of Experimental & Clinical Cancer Research : CR·Udensi K Udensi, Paul B Tchounwou
Feb 11, 2015·Medical Science Monitor Basic Research·Kuan LiuXing Wu
Feb 17, 2015·International Journal of Microbiology·María Elena ValverdeOctavio Paredes-López
Mar 27, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Abidemi J AkindeleAjit K Saxena

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Citations

Nov 20, 2018·Anti-cancer Agents in Medicinal Chemistry·Rafa S AlmeerAhmed E Abdel Moneim
Aug 24, 2019·Current Pharmaceutical Biotechnology·Islam M El-GarawaniEbtesam Nafie
Aug 1, 2019·Toxicology Mechanisms and Methods·Islam M El-GarawaniAhmed E Abdel Moneim
Sep 4, 2019·Organic & Biomolecular Chemistry·Rachel M Gillard, Margaret A Brimble
Mar 28, 2019·Anti-cancer Agents in Medicinal Chemistry·Islam El-GarawaniSamar Almeldin
May 1, 2021·BMC Complementary Medicine and Therapies·Rafa S AlmeerGadah I AlBasher
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Débora Cássia Vieira GomesAna Amélia de Carvalho Melo Cavalcante

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