Ganoderma lucidum polysaccharide improves rat DSS-induced colitis by altering cecal microbiota and gene expression of colonic epithelial cells

Food & Nutrition Research
Jinli XieXichun Peng

Abstract

The effects of β-glucan on colitis mice are contradictory in previous reports. As a result, it is still unclear whether there is an anti-colitis effect in Ganoderma lucidum polysaccharide (GLP), which is mainly composed of β-glucan. Moreover, the association between GLP function and gut microbiota remains to be elucidated. This study aimed to investigate whether GLP consumption improved rat dextran sodium sulfate (DSS)-induced colitis by regulating gut microbiota and altering colonic epithelial expression. The disease activity index (DAI) scores and the cecal short chain fatty acid (SCFA) levels of DSS-induced colitis rats fed with a GLP diet (Group GLP, n = 6) and a control diet (Group Con, n = 6) were investigated and analyzed. Moreover, the profiles of gut microbiota and colonic epithelial expression were analyzed using metagenomics and transcriptomics. GLP consumption significantly lowered animal DAI scores by producing more SCFAs by increasing SCFA-producing bacteria such as Ruminococcus_1 and reducing pathogens such as Escherichia-Shigella in both the small intestine and cecum of rat. Moreover, GLP consumption regulated 11 genes, including six upregulated (Ccl5, Cd3e, Cd8a, Il21r, Lck, and Trbv) and five downregulated (Cc...Continue Reading

References

Nov 4, 2000·Immunogenetics·B Y AlabyevA V Taranin
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei-Ting HungWen-Bin Yang
Jul 29, 2010·FEMS Microbiology Ecology·Christophe ChassardAnnick Bernalier-Donadille
Nov 3, 2010·Journal of Gastroenterology and Hepatology·In-Wha KimJin-Ho Kim
Jan 8, 2011·The American Journal of Chinese Medicine·Zengtao XuYitao Wang
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Apr 11, 2012·Carbohydrate Research·Qun DongKan Ding
Apr 16, 2013·Gastroenterology·Siew C NgUNKNOWN Asia–Pacific Crohn's and Colitis Epidemiologic Study (ACCESS) Study Group
Apr 24, 2013·Nutrients·Joanne Slavin
Jan 16, 2014·Autoimmunity Reviews·Karsten ConradMartin W Laass
Feb 11, 2014·Current Protocols in Immunology·Benoit ChassaingMatam Vijay-Kumar
Sep 11, 2014·World Journal of Gastroenterology : WJG·Rok Orel, Tina Kamhi Trop
Mar 12, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Paul M NguyenMatthias Ernst
Sep 2, 2015·ILAR Journal·Christoph BeckerStefan Wirtz
Oct 27, 2015·Nutrition Research·Sigrid E M HeinsbroekWouter J de Jonge
Dec 15, 2015·Basic & Clinical Pharmacology & Toxicology·María Llorián-SalvadorAna Baamonde
Mar 1, 2016·Frontiers in Microbiology·David Ríos-CoviánNuria Salazar
Nov 9, 2016·Inflammatory Bowel Diseases·Kozue TakeshitaTakanori Kanai
Dec 9, 2016·Environmental Microbiology·Petra Louis, Harry J Flint
Dec 14, 2016·Gastroenterology·Jean-Frederic Colombel, Uma Mahadevan
Aug 10, 2017·Frontiers in Physiology·Núria MachLéa Lansade
Nov 7, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maneli AminshahidiFereshteh Fani

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Citations

Apr 23, 2020·International Journal of Molecular Sciences·Siriluck PonsuksiliKlaus Wimmers
Oct 6, 2020·Journal of Food Biochemistry·Xingyao LongKun-Young Park
Apr 12, 2021·Carbohydrate Polymers·Andrea Caroline RuthesMarcello Iacomini

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Methods Mentioned

BETA
dissection
PCR
electrophoresis
RNA-seq

Software Mentioned

R
Empirical Analysis of Digital Gene Expression in R ( EdgeR )
Goatools
KOBAS
RSEM
QIIME Quantitative Insights into Microbial Ecology
Bowtie2
Ensembl
SPSS
Sickle

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