Gap junction channel gating at bass retinal electrical synapses

Visual Neuroscience
C Lu, D G McMahon

Abstract

To further characterize the properties of retinal horizontal cell electrical synapses, we have studied the gating characteristics of gap junctions between cone-driven horizontal cells from the hybrid striped bass retina using double whole-cell voltage-clamp techniques. In a total of 105 cell pairs, the macroscopic conductance ranged from 0.4-100 nS with most cell pairs exhibiting junctional conductances between 10 and 30 nS. The junctional current-voltage relationship showed that peak or instantaneous currents (Iinst) were linear within the Vj range of +/- 100 mV and that steady-state junctional currents (Iss) exhibited rectification with increasing voltage beginning around +/- 30-40 mV Vj. The normalized junctional current-voltage relationship was well fit by a two-state Boltzmann distribution, with an effective gating charge of 1.9 charges/channel, a half-maximal voltage of approximately +/- 55 mV, and a normalized residual conductance of 0.28. The decay of junctional current followed a single exponential time course with the time constant decreasing with increasing Vj. Recovery of junctional current from voltage-dependent inactivation takes about 1 s following a pulse of 80 mV, and is about five times slower than the inactiv...Continue Reading

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Citations

Mar 9, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peter H LiJulie L Schnapf
Apr 16, 1998·Neuron·I H BrivanlouM Meister
Jan 15, 2013·Progress in Retinal and Eye Research·Béla VölgyiRóbert Gábriel
Dec 8, 2004·Progress in Brain Research·I FahrenfortM Kamermans
Oct 21, 2003·The Journal of Comparative Neurology·Mark PottekReto Weiler
Jul 16, 2003·Pflügers Archiv : European journal of physiology·Rieko SakaiRobert Weingart
May 12, 2001·Science·M KamermansR Weiler

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