Gap junction channels coordinate the propagation of intercellular Ca2+ signals generated by P2Y receptor activation

Glia
Sylvia O SuadicaniEliana Scemes

Abstract

Astrocytes express gap junction proteins and multiple types of P2Y receptors (P2YRs) that contribute to the propagation of intercellular Ca(2+) waves (ICW). To gain access to the role played by gap junctional communication in ICW propagation generated by P2YR activation, we selectively expressed P2Y(1,2,4)R subtypes and Cx43 in the human 1321N1 astrocytoma cell line, which lacks endogenous P2 receptors. Fluorescence recovery after photobleaching revealed that 1321N1 cells are poorly dye-coupled and do not propagate ICW. Forced expression of Cx43 in 1321N1 cells (which did not show functional hemichannels) increased dye coupling and allowed short-range ICW transmission that was mainly mediated by intercellular diffusion of Ca(2+) generated in the stimulated cells. Astrocytoma clones expressing each of the P2YR subtypes were also able to propagate ICWs that were likely dependent on IP(3) generation. These waves exhibited properties particular to each P2YR subtype. Co-expression of eGFP-hCx43 and P2Y(1)R modified the properties of P2Y(1)R-generated ICW to those characteristics of P2Y(2)R. Increased coupling in P2Y(4)R clones induced by expression of eGFP-hCx43 abolished the ICWs observed in uncoupled P2Y(4)R clones. No changes in ...Continue Reading

References

Jul 1, 1992·The Journal of Cell Biology·A C CharlesM J Sanderson
Jun 1, 1992·Neuron·S Finkbeiner
Feb 1, 1991·Cell Calcium·A H Cornell-Bell, S M Finkbeiner
Jul 1, 1988·Trends in Neurosciences·S B KaterJ Connor
Jul 1, 1993·The Journal of General Physiology·L Ebihara, E Steiner
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E ParrJ T Turner
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E ParrJ T Turner
May 1, 1996·Molecular and Cellular Neurosciences·A C CharlesR F Tyndale
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·T D HassingerS B Kater
Sep 30, 1998·The European Journal of Neuroscience·A AraqueP G Haydon
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L CotrinaM Nedergaard
May 14, 1999·Trends in Neurosciences·A AraqueP G Haydon
Aug 24, 1999·British Journal of Pharmacology·E R Lazarowski, T K Harden
Apr 7, 2000·Brain Research. Brain Research Reviews·J I Nagy, J E Rash
Apr 7, 2000·Brain Research. Brain Research Reviews·R DermietzelD C Spray
May 4, 2000·Molecular Biology of the Cell·K PaemeleireM J Sanderson
Oct 18, 2000·Journal of Neurochemistry·A I JiménezM T Miras-Portugal
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·H Zimmermann
Feb 17, 2001·Microscopy Research and Technique·T FryM J Sanderson
Mar 21, 2001·Nature Reviews. Neuroscience·P G Haydon
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·J Lippincott-SchwartzA Kenworthy
Jun 21, 2001·Molecular Neurobiology·E Scemes
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory ArcuinoMaiken Nedergaard
Feb 1, 2003·Trends in Pharmacological Sciences·Maria P AbbracchioGeoffrey Burnstock
Sep 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jorge E ContrerasMichael V L Bennett

❮ Previous
Next ❯

Citations

Aug 1, 2007·The Journal of Membrane Biology·Dumitru A IacobasEliana Scemes
Oct 15, 2008·Pflügers Archiv : European journal of physiology·Eliana ScemesPaolo Meda
May 1, 2012·Purinergic Signalling·Heike FrankePeter Illes
Jul 19, 2008·Neuron Glia Biology·Eliana ScemesDavid C Spray
May 17, 2007·The Journal of General Physiology·David N Bowser, Baljit S Khakh
Sep 30, 2010·The Journal of General Physiology·Stuart E SamuelsKenneth J Muller
Dec 9, 2008·Tissue Engineering. Part a·George Kirmizidis, Mark A Birch
Nov 28, 2006·Journal of Cellular and Molecular Medicine·A Verkhratsky, E C Toescu
Jun 22, 2006·Acta Pharmacologica Sinica·Alexei Verkhratsky
Jul 26, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Giulia CalendaKelvin P Davies
Mar 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peng YueBaofeng Yang
Feb 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sylvia O SuadicaniEliana Scemes
Oct 2, 2009·Chaos·Minchul Kang, Hans G Othmer
Apr 24, 2012·Journal of Environmental and Public Health·Gerry K Schwalfenberg
Nov 28, 2013·Scientifica·Paolo Meda
Nov 6, 2009·Cellular and Molecular Bioengineering·Diana YuGabriel A Silva
Jan 5, 2011·International Journal of Endocrinology·Stuart A HamiltonCarol L Wagner
Jun 16, 2010·Brain Research Reviews·Alexei VerkhratskyJosé J Rodríguez
Feb 11, 2010·Neurochemistry International·Alexei Verkhratsky
May 1, 2007·Experimental Physiology·Hao-Liang Xu, Dale A Pelligrino
Oct 20, 2015·Ageing Research Reviews·Manju Tewari, Pankaj Seth
Aug 1, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Catheleyne D'hondtBernard Himpens
Jul 21, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ying Zhang, Michael Levin
Jun 15, 2006·Movement Disorders : Official Journal of the Movement Disorder Society·Fredricka C Martin, Adrian Handforth
Sep 29, 2006·Glia·Eliana Scemes, Christian Giaume
Jul 11, 2008·The Journal of Comparative Neurology·Hiromi Takahashi-IwanagaKishio Furuya
Dec 17, 2014·Pharmacology Research & Perspectives·Yuling ChiVictor L Schuster
Jan 24, 2012·Biochimica Et Biophysica Acta·Alberto Baroja-MazoPablo Pelegrín
Aug 16, 2011·Biochimica Et Biophysica Acta·Antonio Rodríguez-SinovasDavid Garcia-Dorado
Oct 11, 2005·Biophysical Journal·Dumitru A IacobasEliana Scemes
Nov 4, 2009·Biochimica Et Biophysica Acta·Brynjar FossØystein Bruserud
May 6, 2014·Frontiers in Computational Neuroscience·Jules LallouetteHugues Berry
Feb 13, 2019·International Journal of Molecular Sciences·Yke Jildouw ArnoldussenShanbeh Zienolddiny
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Sep 22, 2017·Pharmacological Reviews·Luc LeybaertRainer Schulz
Jul 20, 2012·Physiological Reviews·Luc Leybaert, Michael J Sanderson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

Journal of Neurochemistry
Christopher M AndersonRaymond A Swanson
Proceedings of the National Academy of Sciences of the United States of America
Gregory ArcuinoM Nedergaard
© 2021 Meta ULC. All rights reserved