Gap Junction Coding Innexin in Lymnaea stagnalis : Sequence Analysis and Characterization in Tissues and the Central Nervous System

Frontiers in Synaptic Neuroscience
Brittany A MersmanFenglian Xu

Abstract

Connections between neurons called synapses are the key components underlying all nervous system functions of animals and humans. However, important genetic information on the formation and plasticity of one type, the electrical (gap junction-mediated) synapse, is understudied in many invertebrates. In the present study, we set forth to identify and characterize the gap junction-encoding gene innexin in the central nervous system (CNS) of the mollusk pond snail Lymnaea stagnalis. With PCR, 3' and 5' RACE, and BLAST searches, we identified eight innexin genes in the L. stagnalis genome, named Lst Inx1-Lst Inx8. Phylogenetic analysis revealed that the L. stagnalis innexin genes originated from a single copy in the common ancestor of molluskan species by multiple gene duplication events and have been maintained in L. stagnalis since they were generated. The paralogous innexin genes demonstrate distinct expression patterns among tissues. In addition, one paralog, Lst Inx1, exhibits heterogeneity in cells and ganglia, suggesting the occurrence of functional diversification after gene duplication. These results introduce possibilities to study an intriguing potential relationship between innexin paralog expression and cell-specific f...Continue Reading

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Datasets Mentioned

BETA
MN480803
DRX001464

Methods Mentioned

BETA
electron microscopy
PCR
dissection
RNA-seq
electrophoresis

Software Mentioned

MEGA
BLAST
featureCounts
ExPASy
TBLASTN
BLASTP
ImageJ
TMHMM Server
Coffee

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