Gap junction currents in cultured muscle cells from human myometrium

American Journal of Obstetrics and Gynecology
H MiyoshiR E Garfield

Abstract

The electrophysiologic properties of gap junctions between human myometrial smooth muscle cells were studied. Double whole-cell patch clamp recordings were made on pairs of cells from primary cultures of myometrial cells from women undergoing cesarean section. Macroscopic gap junction currents were measured as the change in current in a cell held at a constant voltage while the other member of a pair was subjected to a test pulse of voltage. The blockade by halothane was examined. Mean junctional conductance between pairs of cells was 23 +/- 14 nanosiemens (n = 57). Instantaneous gap junction conductance was constant as a function of transjunctional voltage. For transjunctional voltages of < or = 50 mV, currents were constant during a 5-second test pulse. For larger voltages, however, the currents showed a time-dependent decay. The currents were blocked completely and reversibly with 3.5 mmol/L halothane. Single-channel conductances of 60 picosiemens and 15 picosiemens were observed. This first study of gap junction currents in human myometrial cells confirms that connexin43 is the major functional constituent. Functional studies of myometrial gap junction channels may suggest new strategies for controlling uterine contractility.

References

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Citations

Feb 4, 2014·Computational and Mathematical Methods in Medicine·Hinke de LauS Guid Oei
Oct 25, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Chen XuXin Ni
Apr 21, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Andrew M BlanksSteven Thornton
Sep 6, 2012·Journal of Cellular and Molecular Medicine·Gilles Bru-MercierAnatoly Shmygol
Nov 30, 2012·IEEE Transactions on Bio-medical Engineering·Malunoud HassanBrynjar Karlsson
Apr 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Monali Wakle-PrabagaranSarah K England
Jul 8, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Gerald M Kidder, Elke Winterhager
Mar 21, 2002·American Journal of Obstetrics and Gynecology·Brian T PierceLisa M Pierce
May 17, 2013·The Journal of Clinical Endocrinology and Metabolism·Chen XuDavid M Olson
Oct 17, 2002·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·H EswaranC L Lowery
Jan 19, 2011·Acta Obstetricia Et Gynecologica Scandinavica·Miha LucovnikRobert E Garfield
Apr 5, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·George J ChristKarl-Erik Andersson
Mar 10, 1999·The American Journal of Physiology·M D Frame
Jul 22, 2019·Best Practice & Research. Clinical Obstetrics & Gynaecology·Thomas Drew, Mrinalini Balki
Dec 15, 2010·American Journal of Obstetrics and Gynecology·Miha LucovnikRobert E Garfield
Sep 3, 2021·Biophysics Reviews·Sara RussoManuel Ortigueira

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