Gap junction mediated miRNA intercellular transfer and gene regulation: A novel mechanism for intercellular genetic communication

Scientific Reports
Liang ZongHong-Bo Zhao

Abstract

Intercellular genetic communication is an essential requirement for coordination of cell proliferation and differentiation and has an important role in many cellular processes. Gap junction channels possess large pore allowing passage of ions and small molecules between cells. MicroRNAs (miRNAs) are small regulatory RNAs that can regulate gene expression broadly. Here, we report that miRNAs can pass through gap junction channels in a connexin-dependent manner. Connexin43 (Cx43) had higher permeability, whereas Cx30 showed little permeability to miRNAs. In the tested connexin cell lines, the permeability to miRNAs demonstrated: Cx43 > Cx26/30 > Cx26 > Cx31 > Cx30 = Cx-null. However, consistent with a uniform structure of miRNAs, there was no significant difference in permeability to different miRNAs. The passage is efficient; the miRNA level in the recipient cells could be up to 30% of the donor level. Moreover, the transferred miRNA is functional and could regulate gene expression in neighboring cells. Connexin mutation and gap junctional blockers could eliminate this miRNA intercellular transfer and gene regulation. These data reveal a novel mechanism for intercellular genetic communication. Given that connexin expression is c...Continue Reading

References

Jan 1, 1996·Antisense & Nucleic Acid Drug Development·R M HudziakD D Weller
Jun 1, 1997·Antisense & Nucleic Acid Drug Development·J Summerton, D Weller
Feb 13, 2002·Quarterly Reviews of Biophysics·A L Harris
May 28, 2003·Biochemical and Biophysical Research Communications·Martina BeltramelloPaola D'Andrea
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Jul 13, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y ChenG A Altenberg
Jul 23, 2005·The Journal of Physiology·V ValiunasP R Brink
Jul 7, 2007·American Journal of Physiology. Cell Physiology·Sabrina W YumSteven S Scherer
Sep 29, 2007·Biochemical and Biophysical Research Communications·Ernst J WolvetangKenneth S Zuckerman
Aug 1, 2008·Nature·Daehyun BaekDavid P Bartel
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
May 13, 2009·Biochemical and Biophysical Research Communications·Yunfeng WangXi Lin
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Sep 16, 2010·Cancer Research·Mark KatakowskiMichael Chopp
Nov 3, 2010·Molecular and Cellular Neurosciences·Junxian ZhangSabrina W Yum
Jan 20, 2011·Nature Reviews. Genetics·Eric Huntzinger, Elisa Izaurralde
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Francisco J del Castillo, Ignacio del Castillo
Sep 3, 2011·Current Cancer Therapy Reviews·Larissa A GregoryPranela Rameshwar
Oct 12, 2011·Biochimica Et Biophysica Acta·Peter R BrinkIra S Cohen
Feb 4, 2012·Circulation Research·Eva van RooijArthur A Levin
Apr 19, 2012·Nature Reviews. Molecular Cell Biology·Maria Mittelbrunn, Francisco Sánchez-Madrid
Apr 17, 2013·Cellular and Molecular Life Sciences : CMLS·Ivan ContePaola Bovolenta
Jun 16, 2015·Frontiers in Cellular Neuroscience·Jeffrey C Wingard, Hong-Bo Zhao

❮ Previous
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Citations

Sep 24, 2016·Nature Reviews. Cancer·M Teresa Villanueva
Nov 4, 2016·Nature Reviews. Cancer·Trond AasenDale W Laird
Nov 12, 2016·Current Hypertension Reports·Miruna NemeczAdriana Georgescu
Apr 2, 2017·Basic Research in Cardiology·Ashish Kumar GadicherlaLuc Leybaert
Feb 6, 2017·Molecular Cancer·Catharina MelzerRalf Hass
Aug 11, 2017·Antioxidants & Redox Signaling·Amro Elgheznawy, Ingrid Fleming
Mar 1, 2018·Cellular and Molecular Life Sciences : CMLS·Pengbin YinPeifu Tang
Dec 7, 2017·Frontiers in Cardiovascular Medicine·Solenne Paiva, Onnik Agbulut
Jan 10, 2018·Frontiers in Molecular Neuroscience·Francisco J Del Castillo, Ignacio Del Castillo
Jul 31, 2018·Traffic·Heiko Lemcke, Robert David
Nov 27, 2019·Cells·Shahzad Nawaz SyedBernhard Brüne
Jul 1, 2017·Molecular Neurobiology·Irene Faravelli, Stefania Corti
Aug 22, 2017·Frontiers in Molecular Neuroscience·Dominique ThuringerCarmen Garrido
Sep 6, 2018·Cold Spring Harbor Perspectives in Medicine·Fabio Mammano
Oct 6, 2018·Heart Failure Reviews·Guangze ZhaoDecheng Yang
Oct 24, 2017·Current Molecular Biology Reports·Lilian I PlotkinHannah M Davis
Nov 2, 2016·Journal of Cell Science·Srikanth R PolusaniBruce J Nicholson
Apr 27, 2018·International Journal of Molecular Sciences·Silvana ValdebenitoEliseo Eugenin
Jun 23, 2019·Cancers·Anna-Laura PotthoffMatthias Schneider
Aug 26, 2016·Endocrine Regulations·K VoglovaIveta Herichova
Oct 18, 2020·International Journal of Molecular Sciences·Andrés TittarelliFlavio Salazar-Onfray
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia
Mar 2, 2021·Neural Regeneration Research·Gergely SzarkaTamás Kovács-Öller
Mar 5, 2021·Molecular and Cellular Biochemistry·Shuhei FukudaKen-Ichi Nakahama
Apr 1, 2021·Toxicology Mechanisms and Methods·Jeremy GingrichAlmudena Veiga-Lopez
Jun 3, 2021·International Journal of Molecular Sciences·Rosalinda MadonnaLetizia Mattii
May 21, 2021·Carcinogenesis·James E Trosko
Mar 13, 2020·Molecular Therapy. Nucleic Acids·Jaitip TipaneeThierry VandenDriessche

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Methods Mentioned

BETA
fluorescence-activated cell sorting
transfection
PCR
Assay

Software Mentioned

NIH imageJ
Photoshop
SigmaPlot
SPSS

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