Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo

Nature Communications
Maria KotiniRoberto Mayor

Abstract

Connexins are the primary components of gap junctions, providing direct links between cells under many physiological processes. Here, we demonstrate that in addition to this canonical role, Connexins act as transcriptional regulators. We show that Connexin 43 (Cx43) controls neural crest cell migration in vivo by directly regulating N-cadherin transcription. This activity requires interaction between Cx43 carboxy tail and the basic transcription factor-3, which drives the translocation of Cx43 tail to the nucleus. Once in the nucleus they form a complex with PolII which directly binds to the N-cadherin promoter. We found that this mechanism is conserved between amphibian and mammalian cells. Given the strong evolutionary conservation of connexins across vertebrates, this may reflect a common mechanism of gene regulation by a protein whose function was previously ascribed only to gap junctional communication.

References

Oct 1, 1992·The Journal of Cell Biology·R A MeyerR G Johnson
Jan 1, 1991·Methods in Cell Biology·R M Harland
Feb 17, 2001·Development Genes and Evolution·A BorchersD Wedlich
Sep 29, 2001·Molecular Pathology : MP·N GuptaR R Weichselbaum
Aug 19, 2003·Developmental Biology·Dominique AlfandariDouglas W DeSimone
Dec 18, 2003·Gene Expression Patterns : GEP·Shana R Fawcett, M W Klymkowsky
Jun 24, 2004·The Journal of Biological Chemistry·Christine T FuChristian C Naus
Feb 5, 2005·Developmental Cell·Martin CheungJames Briscoe
Apr 29, 2005·Development·Jaime De CalistoRoberto Mayor
May 2, 2006·Advances in Cardiology·Ben N G Giepmans
Mar 16, 2007·Differentiation; Research in Biological Diversity·Teun P de BoerMarcel A G van der Heyden
Apr 13, 2007·Molecular Biology of the Cell·Ping DaiTetsuro Takamatsu
Apr 28, 2007·Progress in Biophysics and Molecular Biology·Elissavet KardamiDavid C Spray
Jun 5, 2008·Nature Reviews. Molecular Cell Biology·Tatjana Sauka-Spengler, Marianne Bronner-Fraser
Dec 17, 2008·Nature·Carlos Carmona-FontaineRoberto Mayor
Jul 21, 2010·Developmental Cell·Eric TheveneauRoberto Mayor
Jul 13, 2011·Methods in Molecular Biology·Eric Theveneau, Roberto Mayor
Aug 3, 2012·Cell and Tissue Research·Jader NonesAndréa Gonçalves Trentin
Sep 5, 2012·Current Opinion in Cell Biology·Eric Theveneau, Roberto Mayor
Sep 8, 2012·Methods in Molecular Biology·Robert C AkkersGert Jan C Veenstra
Sep 22, 2012·BMC Research Notes·Ivan DimauroMalcolm J Jackson
Mar 28, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chao-Jie WangXiao-Feng Sun
May 16, 2013·Development·Roberto Mayor, Eric Theveneau
May 29, 2013·The Journal of Cell Biology·Elias H BarrigaRoberto Mayor
May 3, 2014·Cold Spring Harbor Protocols·George E Gentsch, James C Smith
Jun 3, 2014·Cell Communication and Signaling : CCS·Clàudia Salat-CanelaTrond Aasen

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Citations

Feb 6, 2019·Cancers·Randall J Ruch
Apr 6, 2019·The Journal of Comparative Neurology·Elena IvanovaBotir T Sagdullaev
Mar 1, 2019·Oncogene·Trond AasenArantxa Tabernero
Jan 23, 2020·Journal of Cell Science·Karyn Jourdeuil, Lisa A Taneyhill
Aug 11, 2020·The Journal of Cell Biology·Antoine A KhalilPeter Friedl
Aug 25, 2020·Investigative Ophthalmology & Visual Science·Felix YemanyiVijay Krishna Raghunathan
Sep 11, 2019·Cellular and Molecular Life Sciences : CMLS·Max Zachrisson TotlandEdward Leithe
Apr 17, 2019·Journal of Cell Science·Adam Shellard, Roberto Mayor
Mar 14, 2020·Journal of Cardiovascular Development and Disease·Michael J Zeitz, James W Smyth
Jan 17, 2020·F1000Research·Kathleen J GreenJoshua A Broussard
Apr 17, 2019·Cancers·Joanna GemelEric C Beyer
Jan 23, 2020·International Journal of Molecular Sciences·Dongsheng Jiang, Yuval Rinkevich
Feb 13, 2020·Frontiers in Immunology·Gilson Gonçalves Dos SantosMaripat Corr
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Isabel Martinez-Garay
Nov 6, 2020·Journal of Bone and Mineral Metabolism·Iván Nadir Camal RuggieriSara Feldman
Sep 13, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Asli AdakGulistan Mese
Jan 29, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·Li WanYuval Rinkevich
Oct 3, 2020·Molecular Cancer Research : MCR·Jessica A SmartEdward J Hartsough
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia
Mar 7, 2021·International Journal of Molecular Sciences·Takanori YamaguchiMotomu Shimaoka
May 1, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kai ZhangXiao-Yuan Mao
May 1, 2021·International Journal of Molecular Sciences·Antonio Rodríguez-SinovasIgnacio Ferreira-González
May 21, 2021·Carcinogenesis·James E Trosko
Jul 25, 2021·International Journal of Molecular Sciences·Christoph KoeppleCor de Wit
Aug 26, 2021·Trends in Cell Biology·Dale W Laird, Paul D Lampe
Aug 28, 2021·International Journal of Molecular Sciences·Arsalan Ul HaqPaolo Di Nardo
Aug 28, 2021·Micromachines·Arsalan Ul HaqFabio De Matteis
Jul 30, 2021·The Journal of Clinical Investigation·Tori LhommeVincent Prevot

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Methods Mentioned

BETA
co-immunoprecipitation
immunoprecipitation
PCR
ChIP
electrophoresis
transfection
reversed phase chromatography

Software Mentioned

MaxQuant
Leica IM50
VENUS
ElemeNT
LAS
AF
Mascot
GraphPad
ImageJ Plugins
Prism6

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