Gap junctions in Malpighian tubules of Aedes aegypti

The Journal of Experimental Biology
Xing-He WengK W Beyenbach

Abstract

We present electrical, physiological and molecular evidence for substantial electrical coupling of epithelial cells in Malpighian tubules via gap junctions. Current was injected into one principal cell of the isolated Malpighian tubule and membrane voltage deflections were measured in that cell and in two neighboring principal cells. By short-circuiting the transepithelial voltage with the diuretic peptide leucokinin-VIII we largely eliminated electrical coupling of principal cells through the tubule lumen, thereby allowing coupling through gap junctions to be analyzed. The analysis of an equivalent electrical circuit of the tubule yielded an average gap-junction resistance (R(gj)) of 431 kOmega between two cells. This resistance would stem from 6190 open gap-junctional channels, assuming the high single gap-junction conductance of 375 pS found in vertebrate tissues. The addition of the calcium ionophore A23187 (2 micromol l(-1)) to the peritubular Ringer bath containing 1.7 mmol l(-1) Ca(2+) did not affect the gap-junction resistance, but metabolic inhibition of the tubule with dinitrophenol (0.5 mmol l(-1)) increased the gap-junction resistance 66-fold, suggesting the regulation of gap junctions by ATP. Lucifer Yellow injecte...Continue Reading

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Citations

Feb 19, 2009·Acta Physiologica·G Burnstock, A Verkhratsky
Aug 5, 2011·Journal of Virology·N K MarzianoM W Turnbull
Dec 8, 2009·Annual Review of Entomology·Klaus W BeyenbachJulian A T Dow
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Aug 5, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter M PiermariniKlaus W Beyenbach
Jan 1, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter M PiermariniKlaus W Beyenbach
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Apr 28, 2011·Journal of Insect Science·Kaijun Luo, Matthew W Turnbull
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Dec 29, 2011·Journal of Insect Physiology·Julian A T Dow
Dec 31, 2015·Tissue Barriers·Klaus W BeyenbachJerod Denton
Jan 15, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Travis L CalkinsPeter M Piermarini
May 3, 2019·The Journal of Experimental Biology·Klaus W Beyenbach
Jul 11, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paula GioinoJuan P Ianowski
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Sep 2, 2016·Developmental Neurobiology·I Martha Skerrett, Jamal B Williams
Nov 11, 2021·Open Biology·Jorge Henrique NevesGlaucia Maria Machado-Santelli

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