Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the spiral limbus of the rat cochlea

Cell Communication & Adhesion
Tatsuya MatsunamiMasahito Oyamada

Abstract

To elucidate the role of the spiral limbus in glucose transport in the cochlea, we analyzed the expression and localization of GLUT1, connexin26, connexin30, and occludin in the spiral limbus of the rat cochlea. GLUT1 and occludin were detected in blood vessels. GLUT1, connexin26, connexin30, and occludin were also expressed in fibrocytes just basal to the supralimbal lining cells. Connexin26 and connexin30 were present among not only these GLUT1-positive fibrocytes but also GLUT1-negative fibrocytes. In vivo glucose imaging using 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG, MW 342) together with Evans Blue Albumin (EBA, MW 68,000) showed that 6-NBDG was rapidly distributed throughout the spiral limbus, whereas EBA was localized only in the vessels. Moreover, the gap junctional uncoupler heptanol inhibited the distribution of 6-NBDG. These findings suggest that gap junctions play an important role in glucose transport in the spiral limbus, i.e., that gap junctions mediate glucose transport from GLUT1-positive fibrocytes to GLUT1-negative fibrocytes in the spiral limbus.

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Citations

Jan 13, 2016·Nature Communications·Brad C BennettMark Yeager
Mar 31, 2011·Hearing Research·Birgit MazurekJohann Gross
Feb 27, 2010·Hearing Research·Wen-Hung WangShuan-Yow Li
Sep 16, 2008·Hearing Research·Kevin K Ohlemiller
Jan 18, 2014·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Shawn M StevensHainan Lang
Mar 2, 2016·International Journal of Molecular Sciences·Xing-Xing ZhouWei-Jia Kong
Mar 15, 2011·The Journal of Membrane Biology·Ioana AlesutanFlorian Lang

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