Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system

Biochemical and Biophysical Research Communications
Raymond C B WongAlice Pébay

Abstract

We investigated the gap junctional properties of human embryonic stem cells (hESC) cultivated in a serum-free system using sphingosine-1-phosphate and platelet-derived growth factor (S1P/PDGF). We compared this condition to hESC grown on Matrigel in mouse embryonic fibroblast conditioned medium (MEF-CM) or unconditioned medium (UM). We show that in all culture systems, hESC express connexins 43 and 45. hESC maintained in S1P/PDGF conditions and hESC grown in presence of MEF-CM are coupled through gap junctions while hESC maintained on Matrigel in UM do not exhibit gap junctional intercellular communication. In this latter condition, coupling was retrieved by addition of noggin, suggesting that BMP-like activity in UM inhibits gap junctional communication. Last, our data indicate that the closure of gap junctions by the decoupling agent alpha-glycyrrhetinic acid increases cell apoptosis and inhibits hESC colony growth. Altogether, these results suggest that gap junctions play an important role in hESC maintenance.

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Citations

Aug 3, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Qianli MengYu Huang
Jul 13, 2007·Stem Cell Reviews·M William LenschThorsten M Schlaeger
Aug 16, 2008·Stem Cell Reviews·Raymond C B WongAlice Pébay
Feb 7, 2009·Cell Death and Differentiation·E DecrockL Leybaert
Feb 20, 2013·Human Molecular Genetics·Qiong KeAndy Peng Xiang
Dec 29, 2007·BMC Genomics·Thomas C SchulzXianmin Zeng
Jan 17, 2013·Journal of Cell Science·Konstantin StoletovRichard L Klemke
Aug 1, 2013·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Feb 18, 2015·Stem Cell Research & Therapy·Renjun ZhuKenneth R Boheler
Nov 10, 2007·Prostaglandins & Other Lipid Mediators·Alice PébayStuart M Pitson
Sep 29, 2007·Biochemical and Biophysical Research Communications·Ernst J WolvetangKenneth S Zuckerman
Sep 5, 2014·Tissue Engineering. Part C, Methods·Sébastien SartYan Li
May 12, 2015·Cell Reports·Masahiro HitomiJustin D Lathia
Mar 12, 2015·Anais Da Academia Brasileira De Ciências·Leandro VairoAntonio C Campos De Carvalho
Mar 14, 2014·Cell Transplantation·Karthyayani RajamaniTzyy-Wen Chiou
Oct 28, 2019·Stem Cells·Jessica L EsseltineDale W Laird
Jun 17, 2014·Journal of Cell Science·Tim Pieters, Frans van Roy
Apr 20, 2021·Laryngoscope Investigative Otolaryngology·Kiminori SatoHirohito Umeno
Jul 7, 2021·Tissue Barriers·Anaclet Ngezahayo, Frederike A Ruhe

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