GAPDH-knockdown reduce rotenone-induced H9C2 cells death via autophagy and anti-oxidative stress pathway

Toxicology Letters
Shao LiangZhang Ping

Abstract

GAPDH, well known for its house-keeping functions, has also been shown to be involved in cell injury, apoptosis and death under conditions of stress such as starvation, chemical injury and oxidative stress. This study examines the effect of GAPDH knockdown on cell injury in response to Rotenone. GAPDH was knocked down in H9C2 cardiomyoblasts using siRNA prior to exposure to rotenone (0 nM, 20 nM, 40 nM and 80 nM). Autophagy was detected by western blot for autophagy proteins (Beclin-1, Atg5, LC-3A/B and p62) and MDC staining for acidic substances. Pro-apoptosis protein and flow cytometry were used to assess cell apoptosis and death and intracellular ATP relative concentration was measured. Oxidant stress was assessed by measuring DCFH-DA, TBARS, GSH and SOD. In this study, GAPDH-knockdown enhanced autophagy in rotenone-induced H9C2 cells, decreased oxidant stress and increased antioxidant pathways; and reduced cell apoptosis and death. Furthermore, GAPDH-knockdown preserved cell energy. siRNA-mediated GAPDH knockdown reduced rotenone-induced H9C2 cell death occurring via autophagy and anti-oxidative stress pathway. This study enriches the understanding of GAPDH pathophysiology role, and provides potential new therapeutic target...Continue Reading

References

Aug 29, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·A DonatiE Bergamini
Sep 17, 2008·Journal of Cellular and Molecular Medicine·Narasimman GurusamyDipak K Das
Jul 18, 2009·Neuron·Tomohiro Nakamura, Stuart A Lipton
Oct 31, 2009·Biochemical and Biophysical Research Communications·Hidemitsu NakajimaTadayoshi Takeuchi
Aug 24, 2010·Cellular Signalling·Carlos TristanAkira Sawa
Jun 7, 2011·Cell Metabolism·Daolin TangMichael T Lotze
Nov 24, 2011·Cardiovascular Research·Sang-Bing Ong, Asa B Gustafsson
Dec 22, 2012·ACS Chemical Neuroscience·Burton J MaderJohn J Shacka
Jan 31, 2013·Neurobiology of Disease·Dongxu ZhaiFang Liu

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Citations

Jan 10, 2016·Free Radical Biology & Medicine·Vladimir F LazarevBoris A Margulis
Mar 30, 2016·Scientific Reports·Rosario Pacheco-MarínVilma Maldonado
Jun 4, 2016·Nature Reviews. Neuroscience·Lorenzo GalluzziGuido Kroemer
May 7, 2020·Pharmaceutics·Vladimir F LazarevBoris A Margulis

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