Gas chromatographic determination of cholesterol and tocopherols in edible oils and fats with automatic removal of interfering triglycerides

Journal of Chromatography. a
E BallesterosM Valcárcel

Abstract

An automated gas chromatographic method for the simultaneous determination of cholesterol, alpha-tocopherol and alpha-tocopheryl acetate in edible oils and fats without derivatization is reported. Interferences from lipid material are avoided by using a continuous system to transesterify triglycerides with potassium methylate in methanol. The precision of the method is 1.9, 2.2 and 3.1% for cholesterol, alpha-tocopherol and alpha-tocopheryl acetate, respectively. The proposed methods was validated by analysing a standard reference material of coconut oil (SRM 1563-2) with good results. The method features a high throughput, minimal sample handling and analyte specificity (lipid material does not interfere).

Citations

Mar 30, 2005·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hisaya Terada, Yukio Tamura
Sep 28, 2012·Nutrients·Zhidong XuRafat A Siddiqui
Apr 15, 2015·Journal of Chemical Biology·Haseeb AhsanWaseem A Siddiqui
Mar 18, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sujitra ThaithetSomchai Lapanantnoppakhun
Jul 25, 2000·Journal of Chromatography. a·S L Abidi
Sep 1, 2005·Journal of Agricultural and Food Chemistry·Edward SzłykAgnieszka Kowalczyk-Marzec

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