Gas chromatographic separation of fatty acid methyl esters on weakly polar capillary columns

Journal of Chromatography. a
Kouhei YamamotoAkira Shibahara

Abstract

It was found that weakly polar columns, routinely used in capillary GC for analyzing sterols, food additives, etc., can also be used for separating fatty acid methyl esters (FAMEs). On these columns, FAMEs elute in the order of their unsaturation. The equivalent chain-length value of methyl 22:6 is below 23.00. This means FAMEs within a carbon chain length, having up to six double bonds, elute before the next (one carbon longer) saturated FAME elutes. Peak identification is easy. Weakly polar columns are compatible in both GC and GC/MS systems.

References

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Citations

Jun 4, 2013·Analytical and Bioanalytical Chemistry·Julian C GodingAmber M Hupp
Jul 15, 2012·Pakistan Journal of Biological Sciences : PJBS·Nina ArtantiHarmastini Sukiman
Jul 31, 2019·Journal of Drug Delivery·Miguel A Lopez-ToledanoFrederick D Sancilio
Jun 16, 2009·Journal of Pharmaceutical and Biomedical Analysis·Alejandro Ruiz-RodriguezElena Ibañez

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