Gas chromatography-mass spectrometry method for the determination of free amino acids as their dimethyl-tert-butylsilyl (TBDMS) derivatives in animal source food

Journal of Agricultural and Food Chemistry
Estefanía Jiménez-MartínTeresa Antequera

Abstract

The suitability of a one-step derivatization procedure using N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide for the simultaneous assay of 22 free amino acids and its application for their analysis in six animal source foods (pork, dry cured ham, chicken stock, fresh cheese, ripened cheese, and dry salted sardine) by GC-MS were studied. All 22 free amino acid derivatives were correctly detected and resolved. Reproducibility (%RSD) of the method was in the range of 1.9-12.2%. Detection and quantitation limits of the analytical procedure ranged from 0.01 to 0.46 mg/100 g dry weight and from 0.02 to 1.55 mg/100 g dry weight, respectively. The calibration curves were linear within the range 0.1-15.0 mg/100 g with correlation coefficient values (R(2)) from 0.9891 to 0.9983. All analyzed food products showed free amino acid contents similar to those found in the scientific literature. The proposed GC-MS method for the determination of free amino acids in animal source food can be used in routine for both analytical and research purposes.

References

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Apr 9, 2010·Meat Science·Trinidad Pérez-PalaciosTeresa Antequera

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Citations

Apr 16, 2015·International Journal of Analytical Chemistry·Trinidad Pérez-PalaciosTeresa Antequera
Jun 10, 2016·The Journal of Nutrition·Stephan van VlietNicholas A Burd
Dec 17, 2014·Chemical Communications : Chem Comm·Xiuhong LuWeili Zhao
Nov 13, 2013·International Journal of Food Sciences and Nutrition·Trinidad Pérez-PalaciosIsabel M P L V O Ferreira
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·David J BealeDaniel A Dias
Dec 6, 2020·Animals : an Open Access Journal From MDPI·Krittaporn V ThanatsangWonnop Visessanguan
Aug 20, 2020·Journal of Pharmaceutical and Biomedical Analysis·Özge Cansın ZekiEmirhan Nemutlu
May 11, 2020·Ultrasonics Sonochemistry·Larissa de Lima AlvesAlexandre José Cichoski

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