Gas chromatography/atmospheric pressure chemical ionization-time of flight mass spectrometry: analytical validation and applicability to metabolic profiling

Analytical Chemistry
Alegría Carrasco-PancorboOleg A Mayboroda

Abstract

Gas Chromatography (GC)-Mass Spectrometry (MS) with Atmospheric Pressure (AP) interface was introduced more than 30 years ago but never became a mainstream technique, mainly because of technical difficulties and cost of instrumentation. A recently introduced multipurpose AP source created the opportunity to reconsider the importance of AP ionization for GC. Here, we present an analytical evaluation of GC/APCI-MS showing the benefits of soft atmospheric pressure chemical ionization for GC in combination with a Time of Flight (TOF) mass analyzer. During this study, the complete analytical procedure was optimized and evaluated with respect to characteristic analytical parameters, such as repeatability, reproducibility, linearity, and detection limits. Limits of detection (LOD) were found within the range from 11.8 to 72.5 nM depending on the type of compound. The intraday and interday repeatability tests demonstrate relative standard deviations (RSDs) of peak areas between 0.7%-2.1% and 3.8%-6.4% correspondingly. Finally, we applied the developed method to the analysis of human cerebrospinal fluid (CSF) samples to check the potential of this new analytical combination for metabolic profiling.

References

Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L PaulingP Cary
Jun 24, 2000·Journal of Chromatography. a·M M van DeurseC A Cramers
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Akira NameraTohru Kojima
Oct 8, 2003·Journal of the American Society for Mass Spectrometry·Anthony W T Bristow, Kenneth S Webb
Dec 25, 2004·Journal of Experimental Botany·John M HalketPeter M Bramley
Sep 27, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lauren MacNeilTruis Smith-Palmer
Sep 28, 2005·Journal of the American Society for Mass Spectrometry·Charles N McEwen, Richard G McKay
Dec 15, 2005·Analytical Chemistry·Jiye AThomas Moritz
Feb 16, 2006·Analytical Chemistry·Maud M KoekThomas Hankemeier
Mar 8, 2006·Rapid Communications in Mass Spectrometry : RCM·Suvi OjanperäIlkka Ojanperä
Oct 13, 2006·Methods in Molecular Biology·Oliver Fiehn, Tobias Kind
Feb 24, 2007·Analytical Biochemistry·Tobias KindRobert H Weiss
Dec 11, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tariq Mohabbat, Barry Drew
May 24, 2008·Trends in Analytical Chemistry : TRAC·Oliver Fiehn
Jul 8, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hannelore KasparPeter J Oefner
Jul 29, 2008·Analytical and Bioanalytical Chemistry·Ralf SchiewekOliver J Schmitz
Sep 16, 2008·Analytica Chimica Acta·Bernd O KellerRandy M Whittal
Oct 8, 2008·Analytical Chemistry·Nina BrennerRisto Kostiainen

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Citations

Dec 4, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donald G RobertsonMichael D Reily
Mar 25, 2014·Journal of the American Society for Mass Spectrometry·María Luz Gómez-PérezAntonia Garrido Frenich
Mar 27, 2012·Metabolomics : Official Journal of the Metabolomic Society·Marek J NogaThomas Hankemeier
Jun 26, 2013·Bioanalysis·Tiziana PacchiarottaOleg A Mayboroda
Jan 6, 2016·Journal of the American Society for Mass Spectrometry·Hendrik KerstenAlexander Makarov
Jul 20, 2010·Journal of Mass Spectrometry : JMS·T PortolésP Hancock
May 20, 2011·Rapid Communications in Mass Spectrometry : RCM·Tania PortolésWilfried M A Niessen
Nov 10, 2014·Analytical and Bioanalytical Chemistry·E Hurtado-FernándezA Carrasco-Pancorbo
Dec 23, 2016·Analytical and Bioanalytical Chemistry·Samuel MesihääIlkka Ojanperä
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·David J BealeDaniel A Dias
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alisa PautovaAlexander Revelsky
Aug 29, 2020·Journal of the American Society for Mass Spectrometry·Christopher J ThompsonPhilippe Schmitt-Kopplin

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