Gas chromatography/chemical ionization triple quadrupole mass spectrometry analysis of anabolic steroids: ionization and collision-induced dissociation behavior

Rapid Communications in Mass Spectrometry : RCM
Michael PoletKoen Deventer

Abstract

The detection of new anabolic steroid metabolites and new designer steroids is a challenging task in doping analysis. Switching from electron ionization gas chromatography triple quadrupole mass spectrometry (GC/EI-MS/MS) to chemical ionization (CI) has proven to be an efficient way to increase the sensitivity of GC/MS/MS analyses and facilitate the detection of anabolic steroids. CI also extends the possibilities of GC/MS/MS analyses as the molecular ion is retained in its protonated form due to the softer ionization. In EI it can be difficult to find previously unknown but expected metabolites due to the low abundance or absence of the molecular ion and the extensive (and to a large extent unpredictable) fragmentation. The main aim of this work was to study the CI and collision-induced dissociation (CID) behavior of a large number of anabolic androgenic steroids (AAS) as their trimethylsilyl derivatives in order to determine correlations between structures and CID fragmentation. Clarification of these correlations is needed for the elucidation of structures of unknown steroids and new metabolites. The ionization and CID behavior of 65 AAS have been studied using GC/CI-MS/MS with ammonia as the reagent gas. Glucuronidated AAS ...Continue Reading

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Citations

Nov 26, 2016·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Dec 7, 2019·Mass Spectrometry Reviews·David J Harvey, Paul Vouros
Jan 2, 2021·Forensic Science International : Synergy·Nicole S Jones, Jeffrey H Comparin
Feb 29, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pieter Van RenterghemPeter Van Eenoo
May 1, 2021·Biomolecules·Michal KaletaMiroslav Strnad

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