Gas chromatography/mass spectrometry based metabolomic study in a murine model of irritable bowel syndrome

World Journal of Gastroenterology : WJG
Lei-Min YuBin Lu

Abstract

To study the role of microbial metabolites in the modulation of biochemical and physiological processes in irritable bowel syndrome (IBS). In the current study, using a metabolomic approach, we analyzed the key metabolites differentially excreted in the feces of control mice and mice with IBS, with or without Clostridium butyricum (C. butyricum) treatment. C57BL/6 mice were divided into control, IBS, and IBS + C. butyricum groups. In the IBS and IBS + C. butyricum groups, the mice were subjected to water avoidance stress (WAS) for 1 h/d for ten days. Gas chromatography/mass spectrometry (GC-MS) together with multivariate analysis was employed to compare the fecal samples between groups. WAS exposure established an appropriate model of IBS in mice, with symptoms of visceral hyperalgesia and diarrhea. The differences in the metabolite profiles between the control group and IBS group significantly changed with the progression of IBS (days 0, 5, 10, and 17). A total of 14 differentially excreted metabolites were identified between the control and IBS groups, and phenylethylamine was a major metabolite induced by stress. In addition, phenylalanine metabolism was found to be the most relevant metabolic pathway. Between the IBS group ...Continue Reading

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Citations

Mar 4, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Jae Soung LeeHyung-Kyoon Choi
May 20, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Sean M BennetStephen J Vanner
Jul 26, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Shanshan NieDongsheng Wang
Jan 13, 2019·Molecular Neurodegeneration·Yaping Shao, Weidong Le
Apr 30, 2020·Metabolites·Joseph S MillerKevin V Hackshaw
Nov 5, 2019·Frontiers in Cellular and Infection Microbiology·Shengtao ZhuShutian Zhang
Sep 29, 2020·Journal of Biomedical Science·Lesha Pretorius, Carine Smith
Nov 13, 2020·Critical Reviews in Analytical Chemistry·P Rodríguez-HernándezV Rodríguez-Estévez
Nov 24, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiaojiao ChenXiufeng Ling

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Methods Mentioned

BETA
PCA
nuclear magnetic resonance

Software Mentioned

Chroma TOF
GraphPad Prism
SPSS
R
SIMCA14
DA
XCMS
MetaboAnalyst
MassHunter Workstation
OPLS

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