Gas chromatography/time-of-flight mass spectrometry based metabonomic approach to differentiating hypertension- and age-related metabolic variation in spontaneously hypertensive rats

Rapid Communications in Mass Spectrometry : RCM
Yihong LuKun Hao

Abstract

Metabonomics is a systematic approach to the study of in vivo metabolic profiles and therefore allows deep insight into and a better understanding of the pathogenesis of disease. To characterize the development of hypertension, a hypertensive animal model, the spontaneously hypertensive rat (SHR), and its normotensive control, the Wistar Kyoto (WKY) rat, were investigated and their blood plasma analyzed using the high-throughput metabolomic tool, gas chromatography/time-of-flight mass spectrometry (GC/TOFMS). A total of 187 peaks were quantitatively determined after deconvolution, and 78 of them were identified. Principal components analysis (PCA) and projection to latent structure partial least-squares discriminant analysis (PLS-DA) were used to process the GC/TOFMS data. The resulting mathematical models were further validated by cross-validation. Plasma compositional differences of many identified compounds showed hypertension-related variation between SHR and WKY rats, and age-related changes from 10 to 18 weeks for both the SHR and WKY rats. These compositional changes involved compounds such as hexadecanoic acid, linoleic acid, oleic acid, stearic acid, 3-hydroxybutyric acid, citric acid, threonic acid, tyrosine, tryptoph...Continue Reading

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Citations

May 22, 2010·Age·Lauri O ByerleyUNKNOWN Louisiana Healthy Aging Study
Nov 18, 2011·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Kazuki AkiraTakao Hashimoto
Jan 6, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Kinza S ShamsiSara E Espinoza
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Dec 17, 2009·Journal of Pharmaceutical and Biomedical Analysis·Kazuki AkiraTakao Hashimoto
Mar 3, 2012·Journal of Proteome Research·Nari SonHyun-Jin Kim
Feb 7, 2017·Experimental Biology and Medicine·Jacek BartosiewiczDariusz Pawlak
Mar 29, 2014·Journal of Hypertension·Sonja B NikolicLindsay M Edwards

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