Gas-liquid chromatography of fecal neutral steriods

Journal of Chromatography
K O GerhardtD J Hentges

Abstract

A method is described for the analysis of fecal neutral steriods with a dual-column gas-liquid chromatography (GLC) system. After saponification of the fecal slurry, the neutral steroids were extracted with hexane. The GLC separation of the compounds and quantitation were achieved by simultaneous injection of the derivatized and derivatized aliquots of the extract onto dual colmuns under identical conditions. The neutral steroids of interest were than identified by matching the retention times with those of known standards, and identification was confirmed by use of an interfaced GLC high-resolution mass spectrometry system. The detection limit was 0.003 mg of steroid/g of fecal slurry. The pricision of the method is illustrated by a relative standard diviation of 2-10% and a recovery of neutral steroids from 73-96%. The method was applied to the determination of fecal neutral steroids in a "High protein diet in colon cancer study". A considerably larger level of coprostanone than of coprostanol was observed. Data on neutral steroids in fecal samples from subjects on different diets are the subject of a separate publication.

References

Dec 1, 1974·The American Journal of Clinical Nutrition·B R MaierD J Hentges
Jun 1, 1973·Journal of the National Cancer Institute·B S Reddy, E L Wynder
Jun 1, 1971·The Journal of Pathology·M J Hill, V C Aries
Feb 1, 1956·Archives of Biochemistry and Biophysics·D L COLEMANC A BAUMANN
Sep 1, 1962·Analytical Biochemistry·W W WELLS, M MAKITA

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Citations

Dec 1, 1978·Mechanisms of Ageing and Development·T R ThomasC W Gehrke
Nov 28, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cindy KunneRonald P J Oude Elferink
Apr 14, 2017·Scientific Reports·Marcela DoktorovaJohan W Jonker

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