Gas-liquid chromatography-tandem mass spectrometry methodology for the quantitation of estrogenic contaminants in bile of fish exposed to wastewater treatment works effluents and from wild populations

Journal of Chromatography. a
Kate A FenlonE M Hill

Abstract

Fish can be exposed to a complex mixture of chemical contaminants arising from the exposure to wastewater treatment works (WwTWs) effluents. Some of these contaminants are estrogenic and have been associated with feminisation of male fish and the presence of populations containing intersex individuals. However the detection of trace levels (ng/L) of estrogenic chemicals surface waters can be difficult and does not give information on the exposure of aquatic organisms to these contaminants. In this study we assessed whether the analysis of estrogenic substances that bioconcentrate in fish bile can be used to detect the exposure of fish to feminising contaminants in receiving waters and effluents, and thus facilitate their monitoring of these substances in aquatic environments. Estrogenic metabolites in bile were deconjugated using enzymatic hydrolysis and partially purified by solid phase extraction. Steroidal and xenoestrogens were derivatized to their trimethylsilyl ethers and quantified by gas-liquid chromatography-mass spectrometry (GC-MS/MS) using multiple reaction monitoring. The method was validated using spiked bile samples from immature female rainbow trout (Oncorhynchus mykiss) as well as bile from sexually mature roac...Continue Reading

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Citations

Nov 4, 2011·Environmental Science & Technology·Pawel RostkowskiElizabeth M Hill
Nov 19, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andrew C Johnson, John P Sumpter
Apr 23, 2013·The Science of the Total Environment·James Gelsleichter, Nancy J Szabo
Jul 29, 2015·The Science of the Total Environment·Oihana RosAsier Vallejo
Jun 2, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·A Jurek, E Leitner
Jan 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Huiyong WangWeiming Li
Apr 13, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·A Jurek, E Leitner
Dec 18, 2015·Dose-response : a Publication of International Hormesis Society·Jone CorralesBryan W Brooks
Mar 17, 2020·Environmental Science. Processes & Impacts·Wiesława RuczyńskaTomasz Nermer
Nov 18, 2018·The Science of the Total Environment·Maria BlancoCinta Porte
Nov 2, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sijia LiuXianming Kong

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