Gas-phase hydrogen/deuterium exchange in a traveling wave ion guide for the examination of protein conformations.

Analytical Chemistry
Kasper D RandJohn R Engen

Abstract

Accumulating evidence suggests that solution-phase conformations of small globular proteins and large molecular protein assemblies can be preserved for milliseconds after electrospray ionization. Thus, the study of proteins in the gas phase on this time scale is highly desirable. Here we demonstrate that a traveling wave ion guide (TWIG) of a Synapt mass spectrometer offers a highly suitable environment for rapid and efficient gas-phase hydrogen/deuterium exchange (HDX). Gaseous ND(3) was introduced into either the source TWIG or the TWIG located just after the ion mobility cell, such that ions underwent HDX as they passed through the ND(3) on the way to the time-of-flight analyzer. The extent of deuterium labeling could be controlled by varying the quantity of ND(3) or the speed of the traveling wave. The gas-phase HDX of model peptides corresponded to labeling of primarily fast exchanging sites due to the short labeling times (ranging from 0.1 to 10 ms). In addition to peptides, gas-phase HDX of ubiquitin, cytochrome c, lysozyme, and apomyoglobin were examined. We conclude that HDX of protein ions in a TWIG is highly sensitive to protein conformation, enables the detection of conformers present on submilliseconds time scales,...Continue Reading

References

Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SuckauF W McLafferty
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·D S GrossE R Williams
Aug 1, 1996·Journal of Mass Spectrometry : JMS·E R Williams
Dec 8, 1998·Rapid Communications in Mass Spectrometry : RCM·K Sannes-LoweryS A Hofstadler
Oct 14, 2000·Annual Review of Physical Chemistry·M F Jarrold
May 30, 2002·Journal of the American Chemical Society·Kathrin BreukerFred W McLafferty
Sep 24, 2004·Rapid Communications in Mass Spectrometry : RCM·Kevin GilesRobert H Bateman
Jan 25, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Frank SobottCarol V Robinson
Jul 16, 2005·Journal of the American Society for Mass Spectrometry·Ethan R BadmanDavid E Clemmer
Jul 19, 2005·Journal of the American Society for Mass Spectrometry·Errol W Robinson, Evan R Williams
Oct 7, 2005·Mass Spectrometry Reviews·Thomas E Wales, John R Engen
Mar 28, 2006·Current Opinion in Structural Biology·Justin L P Benesch, Carol V Robinson
Jul 15, 2006·The Journal of Physical Chemistry. a·Orit Geller, Chava Lifshitz
Jan 4, 2008·Journal of the American Chemical Society·Kasper D RandThomas J D Jørgensen
Aug 9, 2008·Journal of the American Chemical Society·Jingxi PanLars Konermann
Sep 13, 2008·Chembiochem : a European Journal of Chemical Biology·Michal Z SteinbergKathrin Breuker
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kathrin Breuker, Fred W McLafferty
Jul 19, 2008·Annual Review of Analytical Chemistry·Brian C BohrerDavid E Clemmer
May 1, 1994·Journal of the American Society for Mass Spectrometry·M E HemlingS A Carr
Dec 1, 1994·Journal of the American Society for Mass Spectrometry·R R Ogorzalek LooR D Smith

❮ Previous
Next ❯

Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Kasper D RandJeffery M Brown
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Stephen V SciutoLars Konermann
Aug 2, 2011·Analytical Chemistry·F HalgandSonia Longhi
Jan 22, 2013·Analytical Chemistry·Hongli LiHerbert H Hill
Apr 1, 2011·The Journal of Physical Chemistry. B·Brian C BohrerDavid E Clemmer
Jun 30, 2012·Nature Reviews. Drug Discovery·Steven A BerkowitzGraham B Jones
Oct 1, 2014·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Jan 24, 2016·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Dec 2, 2015·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Apr 15, 2011·Journal of Pharmaceutical Sciences·Damian HoudeJohn R Engen
Mar 12, 2016·Journal of the American Society for Mass Spectrometry·Shaolong ZhuDerek J Wilson
Aug 21, 2015·Journal of the American Society for Mass Spectrometry·Mahsan MiladiTouradj Solouki
Jan 22, 2015·Proteomics·Helen S BeestonAlison E Ashcroft
Oct 16, 2012·Biochimica Et Biophysica Acta·L A WoodsA E Ashcroft
Mar 9, 2010·Journal of Molecular Biology·Jing FangPenny J Beuning
Apr 22, 2015·Journal of the American Society for Mass Spectrometry·Mahdiar KhakinejadStephen J Valentine
Feb 19, 2015·Journal of the American Society for Mass Spectrometry·Emily R SchenkFrancisco Fernandez-Lima
Dec 17, 2014·Journal of the American Society for Mass Spectrometry·Gregory C DonohoeStephen J Valentine
Oct 17, 2014·Journal of the American Society for Mass Spectrometry·Khadijeh Rajabi
Jun 21, 2016·Rapid Communications in Mass Spectrometry : RCM·Shaan RashidPaul M Mayer
Aug 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liliana Pedro, Ronald J Quinn
Oct 21, 2016·Journal of the American Society for Mass Spectrometry·Guillaume Van der RestFrédéric Halgand
May 10, 2017·Journal of the American Chemical Society·Erik T JanssonRichard N Zare
Apr 1, 2018·Mass Spectrometry Reviews·Yury KostyukevichEugene Nikolaev
Jan 3, 2015·Physical Chemistry Chemical Physics : PCCP·Khadijeh Rajabi
Nov 22, 2018·Journal of the American Society for Mass Spectrometry·Ulrik H MistarzKasper D Rand
Oct 16, 2018·Rapid Communications in Mass Spectrometry : RCM·Liwen HuangZhong-Ping Yao
Aug 5, 2017·Expert Opinion on Drug Discovery·Glenn R MassonJohn E Burke
Dec 22, 2010·Chemical Society Reviews·Lars KonermannYu-Hong Liu
Feb 19, 2011·Expert Review of Proteomics·Michael J ChalmersPatrick R Griffin
Jan 1, 2015·Journal of Mass Spectrometry : JMS·Christine M FisherScott A McLuckey
Oct 9, 2019·Molecular & Cellular Proteomics : MCP·Ryan J Lumpkin, Elizabeth A Komives
Jun 21, 2017·Scientific Reports·Chris GessnerDionysios P Pantazatos
Jul 16, 2013·Angewandte Chemie·Sebastian TittebrandtWolf D Lehmann
Dec 1, 2019·Molecular & Cellular Proteomics : MCP·Ryan J Lumpkin, Elizabeth A Komives
Dec 13, 2016·Journal of the American Society for Mass Spectrometry·Rita N Straus, Rebecca A Jockusch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.