Gas Vesicle Nanoparticles for Antigen Display

Vaccines
S DasSarma, Priya DasSarma

Abstract

Microorganisms like the halophilic archaeon Halobacterium sp. NRC-1 produce gas-filled buoyant organelles, which are easily purified as protein nanoparticles (called gas vesicles or GVNPs). GVNPs are non-toxic, exceptionally stable, bioengineerable, and self-adjuvanting. A large gene cluster encoding more than a dozen proteins has been implicated in their biogenesis. One protein, GvpC, found on the exterior surface of the nanoparticles, can accommodate insertions near the C-terminal region and results in GVNPs displaying the inserted sequences on the surface of the nanoparticles. Here, we review the current state of knowledge on GVNP structure and biogenesis as well as available studies on immunogenicity of pathogenic viral, bacterial, and eukaryotic proteins and peptides displayed on the nanoparticles. Recent improvements in genetic tools for bioengineering of GVNPs are discussed, along with future opportunities and challenges for development of vaccines and other applications.

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Citations

Jan 11, 2017·Molecular Pharmaceutics·Abhay U AndarShiladitya DasSarma
Jun 17, 2016·Molecular BioSystems·Asier GalánMangesh Bhide
Mar 19, 2016·Environmental Microbiology·R Gary Sawers
Feb 1, 2020·Microbiology Resource Announcements·Brian P AntonShiladitya DasSarma
Dec 4, 2020·Biotechnology Advances·Kevin PfeiferSimon K-M R Rittmann
Jul 16, 2021·Microbiology Resource Announcements·Priya DasSarmaShiladitya DasSarma
Feb 14, 2018·Bioconjugate Chemistry·Alexandra N Tsoras, Julie A Champion
Dec 3, 2021·Microbiology Resource Announcements·Priya DasSarmaShiladitya DasSarma

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Methods Mentioned

BETA
electron microscopy
X-ray
ELISA

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