GAS2L3, a target gene of the DREAM complex, is required for proper cytokinesis and genomic stability

Journal of Cell Science
Patrick WolterStefan Gaubatz

Abstract

The mammalian DREAM complex is a key regulator of cell-cycle-regulated gene transcription and drives the expression of many gene products required for mitosis and cytokinesis. In this study, we characterized GAS2L3, which belongs to the GAS2 family of proteins with putative actin- and microtubule-binding domains as a target gene of DREAM. We found that GAS2L3 localizes to the spindle midzone and the midbody during anaphase and cytokinesis, respectively. Biochemical studies show that GAS2L3 binds to and bundles microtubules as well as F-actin in vitro. Strikingly, the RNAi-mediated knockdown of GAS2L3 results in chromosome segregation defects in multinucleated cells and in cells with multi-lobed nuclei. Likewise, chronic downregulation of GAS2L3 causes chromosome loss and aneuploidy. Time-lapse videomicroscopy experiments in GAS2L3-knockdown cells reveal abnormal oscillation of chromatin and the spindle during cytokinesis. Taken together, our data reveal novel, important roles of GAS2L3 for faithful cell division. Our work thus contributes to the understanding of how DREAM regulates cytokinesis.

References

Jun 1, 1992·The Journal of Cell Biology·C BrancoliniC Schneider
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Apr 10, 1997·Nature·C LengauerB Vogelstein
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Jeffrey M Trimarchi, Jacqueline A Lees
Jun 26, 2002·The Journal of Cell Biology·Cristiana MollinariRobert L Margolis
Oct 22, 2004·Molecular Biology of the Cell·Aaron F StraightTimothy J Mitchison
Nov 13, 2004·The EMBO Journal·Sladjana GagricaStefan Gaubatz
Apr 20, 2005·Oncogene·David Cobrinik
Apr 20, 2005·Oncogene·Desssislava K Dimova, Nicholas J Dyson
Oct 26, 2005·Trends in Cell Biology·Alisa PieknyMichael Glotzer
Feb 21, 2006·Current Opinion in Cell Biology·Shuh Narumiya, Shingo Yasuda
Jun 8, 2006·Annual Review of Biochemistry·Ulrike S EggertChristine M Field
Nov 14, 2006·The Journal of Biological Chemistry·Mark PilkintonOscar R Colamonici
Nov 13, 2007·Nature Cell Biology·Ralf KittlerFrank Buchholz
Dec 26, 2007·Current Biology : CB·Alisa J Piekny, Michael Glotzer
May 17, 2008·Biochemical Society Transactions·Gilles R X Hickson, Patrick H O'Farrell
Jun 11, 2008·The Journal of Cell Biology·Anthony TigheStephen Taylor
Jul 25, 2008·Nature Reviews. Cancer·Deborah L Burkhart, Julien Sage
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sander van den Heuvel, Nicholas J Dyson
Feb 11, 2009·Cell·Patrick SteigemannDaniel W Gerlich
Feb 11, 2009·The Journal of Cell Biology·Alberto TosoAndrew D McAinsh
Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Andrew J Holland, Don W Cleveland
Apr 8, 2010·FEBS Letters·Antonia P Sagona, Harald Stenmark
Jul 7, 2010·The Journal of Cell Biology·Kathleen E Rankin, Linda Wordeman
Aug 25, 2010·Seminars in Cell & Developmental Biology·Alisa J Piekny, Amy Shaub Maddox
May 13, 2011·The Journal of Biological Chemistry·Matthew J StroudChristoph Ballestrem

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Citations

Aug 12, 2014·Nucleic Acids Research·Gerd A MüllerKurt Engeland
Sep 20, 2015·Nucleic Acids Research·Martin FischerKurt Engeland
Aug 19, 2014·Developmental Biology·Yaara SharabyAmit Tzur
Sep 3, 2013·Biochimica Et Biophysica Acta·Yi-Chih WuYijuang Chern
Nov 3, 2010·Seminars in Cell & Developmental Biology·Irene MeyerRalph Gräf
Nov 11, 2017·Cell Death and Differentiation·Kurt Engeland
Nov 21, 2015·Journal of Cell Science·Gemma C GirdlerKatja Röper
Aug 12, 2017·Critical Reviews in Biochemistry and Molecular Biology·Martin Fischer, Gerd A Müller
Nov 20, 2019·The FEBS Journal·Hashim AliMauro Giacca
Sep 25, 2017·Cell Cycle·Marco GründlStefan Gaubatz
Jul 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Sabine StoppStefan Gaubatz
Apr 11, 2019·Scientific Reports·Alicja NazgiewiczChristoph Ballestrem
Sep 14, 2020·Cellular and Molecular Life Sciences : CMLS·Nathalia Meireles Da CostaLuiz Eurico Nasciutti
Oct 27, 2020·Cell Proliferation·Nan ZhangXingjie Gao
Jan 1, 2018·Cell Death and Differentiation·Kurt Engeland

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