Gastric acid induces mucosal H2S release in rats by upregulating mRNA and protein expression of cystathionine gamma lyase

The Journal of Physiological Sciences : JPS
Seyyed Ali MardMohammad Kazem Gharib-Naseri

Abstract

It is well known that hydrogen sulfide (H2S) protects the gastric mucosa against gastric acid and other noxious stimulants by several mechanisms but until now the effect of gastric acid on H2S production has not been evaluated. This study was performed to determine the effect of basal and stimulated gastric acid secretion on mRNA and protein expression of cystathionine gamma lyase (CSE) and cystathionine beta synthase (CBS), and on mucosal release of H2S in rats. Seventy-two male rats were randomly assigned into 9 groups (8 in each)-control, distention, and pentagastrin-induced gastric acid secretion groups. The effects of 15% alcohol solution, propargylglycine (PAG), L-NAME, and pantoprazole were also investigated. Under anesthesia, animals underwent tracheostomy and midline laparotomy. A catheter was inserted into the stomach through the duodenum for gastric washout. At the end of the experiments, the animals were killed and the gastric mucosa was collected to measure H2S concentration and to quantify mRNA expression of CSE and CBS by quantitative real-time PCR, and expression of their proteins by western blot. Basal and stimulated gastric acid secretion increased mucosal levels of H2S, and mRNA and protein expression of CSE....Continue Reading

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K Pantopoulos, M W Hentze
Feb 13, 2001·Trends in Cell Biology·C Bogdan
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Weimin Zhao, Rui Wang
Sep 15, 2004·Diabetes Research and Clinical Practice·Fatemeh Nabavizadeh Rafsanjani, Jalal Vahedian
Jun 6, 2009·The Journal of Pharmacology and Experimental Therapeutics·Jand Venes R MedeirosMarcellus H L P Souza
Jan 27, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J AuerM J M Fischer
Mar 2, 2010·Amino Acids·Hideo Kimura
Jan 25, 2012·Digestive Diseases and Sciences·Seyyed Ali MardMaryam Maleki
Feb 4, 2012·Current Medicinal Chemistry·K TakeuchiS Hayashi
Mar 20, 2012·Digestive Diseases and Sciences·John L Wallace
Mar 12, 2013·Postȩpy higieny i medycyny doświadczalnej·Marcin MagierowskiTomasz Brzozowski
Aug 2, 2013·Arquivos De Gastroenterologia·Jand-Venes Rolim MedeirosMarcellus Henrique Loiola Ponte de Souza
Apr 8, 2014·BioMed Research International·Seyyed Ali MardAli Veisi

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Citations

Oct 27, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S A MardM Badavi
Dec 30, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M Saghazadeh-DezfuliS A Mard
Oct 23, 2019·The Science of the Total Environment·Si-Cheng XingXin-Di Liao
Aug 7, 2021·Frontiers in Pharmacology·Wout VerbeurePeter R van Dijk

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