Gastric epithelial cells secrete a PDGF-like peptide, a potent mitogen for human gastric fibroblasts

Proceedings of the Society for Experimental Biology and Medicine
K WadaM Kasuga

Abstract

To investigate whether gastric epithelial cells secrete growth factors involved in stromal cell growth, we examined the effects of conditioned media obtained from gastric cancer cells on murine BALB/c 3T3 cells and primary cultured human gastric fibroblasts. Conditioned media from MKN-1 gastric cancer cells were applied to a heparin-affinity column. The fraction eluted from the column at 0.4 M NaCl stimulated DNA synthesis and phosphorylation of PDGF alpha-receptors on tyrosine in BALB/c 3T3 cells. The fraction-induced stimulation of DNA synthesis in gastric fibroblasts was more marked than in BALB/c 3T3 cells. However, the fraction failed to stimulate DNA synthesis in CHO-ER cells overexpressing EGF receptors and phosphorylation of PDGF beta-receptors on tyrosine in BALB/c 3T3 cells. Immunoblot analysis of the media confirmed that PDGF-AA-like peptides are released from gastric cancer cells, immortalized gastric epithelial cells, and primary cultured gastric epithelial cells. Anti-PDGF neutralizing antibodies produced only a partial inhibition of 0.4 M NaCl fraction-induced enhanced DNA synthesis. Thus, in addition to PDGF-AA peptide, other bioactive substance(s) are probably released from MKN-1 gastric cancer cells. Our resul...Continue Reading

Citations

Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Thèrése DahlmanKristofer Rubin
Dec 2, 2005·Biology of the Neonate·Melissa D HalpernBohuslav Dvorak
Nov 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melissa D HalpernBohuslav Dvorak
Jan 17, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melissa D HalpernBohuslav Dvorak

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